ZFIN ID: ZDB-FIG-151112-24
Jackson et al., 2015 - Zebrafish Wnt9b Patterns the First Pharyngeal Arch into D-I-V Domains and Promotes Anterior-Medial Outgrowth. American Journal of Molecular Biology   5(3):57-83 Full text @ Am. J. of Molec. Bio.
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 5 Wnt9b morphants exhibit severely reduced dlx2a expression in pharyngeal arches 1 and 3-7. (a)-(d) Anterior to right, top row = dorsal view, bottom row = lateral view. MM control (n = 17) (a), (c) and morphant (n = 20) (b), (d) embryos were aged to 24hpf and stained with dlx2a antisense riboprobe. 60% of morphant embryos exhibited reduced dlx2a expression in the pharyngeal arches compared to controls. Red arrowheads indicate forebrain. Black arrows indicate PA1, black arrowheads indicate PA2, white arrowheads indicate PA3-7. Note that dlx2a expression is mostly reduced in PA1 and PA3-7. Br = brain, E = eye. Size bars = 100 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a AB control Prim-5 forebrain RTPCR
Prim-5 pharyngeal arch 1 RTPCR
Prim-5 pharyngeal arch 2 RTPCR
Prim-5 pharyngeal arch 3-7 RTPCR
AB + MO2-wnt9b standard conditions Prim-5 forebrain ISH
Prim-5 pharyngeal arch 1 ISH
Prim-5 pharyngeal arch 2 ISH
Prim-5 pharyngeal arch 3-7 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-wnt9b standard conditions Prim-5 pharyngeal arch 1 dlx2a expression decreased amount, abnormal
Prim-5 pharyngeal arch 3-7 dlx2a expression decreased amount, abnormal
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. of Molec. Bio.