ZFIN ID: ZDB-FIG-151112-25
Jackson et al., 2015 - Zebrafish Wnt9b Patterns the First Pharyngeal Arch into D-I-V Domains and Promotes Anterior-Medial Outgrowth. American Journal of Molecular Biology   5(3):57-83 Full text @ Am. J. of Molec. Bio.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 6 Wnt9b morphants exhibit disrupted Wnt3a and Wnt5b expression domains. Anterior to left. Left column is Wnt3a expression, right column is Wnt5b expression. (a) MM control (n = 14) and (b) morphant embryos (n = 14) were aged to 28hpf and stained with Wnt3a riboprobe. 71% of morphant embryos exhibited expanded Wnt3a expression in the brain compared to controls. (c) MM control (n = 10) and (d) morphant embryos (n = 9) were aged to 28hpf and stained with Wnt5b riboprobe. 100% of morphant embryos exhibited expanded Wnt5b expression in the tail with reduced Wnt5b expression in the pharyngeal arches compared to controls. (a), (b) Red arrowheads indicate brain expression, black arrows indicate rhombic lip expression. (c), (d) Red arrows indicate the PAs, white arrowheads indicate the fin buds, black arrowheads indicate the tail.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
wnt3a AB control Prim-5 brain ISH
Prim-5 hindbrain ISH
AB + MO2-wnt9b standard conditions Prim-5 brain ISH
Prim-5 hindbrain ISH
wnt5b AB control Prim-5 pectoral fin bud ISH
Prim-5 pharyngeal arch ISH
Prim-5 tail bud ISH
AB + MO2-wnt9b standard conditions Prim-5 pectoral fin bud ISH
Prim-5 pharyngeal arch ISH
Prim-5 tail bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-wnt9b standard conditions Prim-5 brain wnt3a expression increased distribution, abnormal
Prim-5 pectoral fin bud wnt5b expression increased amount, abnormal
Prim-5 pharyngeal arch wnt5b expression decreased amount, abnormal
Prim-5 tail bud wnt5b expression increased amount, abnormal
Prim-5 tail bud wnt5b expression increased distribution, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. of Molec. Bio.