ZFIN ID: ZDB-FIG-151112-26
Jackson et al., 2015 - Zebrafish Wnt9b Patterns the First Pharyngeal Arch into D-I-V Domains and Promotes Anterior-Medial Outgrowth. American Journal of Molecular Biology   5(3):57-83 Full text @ Am. J. of Molec. Bio.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 7 Wnt9b morphants display disrupted Wnt5b and sox9a expression domains in the developing upper jaw. MM control (n = 11) (left column) and morphant (n = 13) (right column) embryos were aged to 48hpf and stained with Wnt5b riboprobe (a)-(f) or sox9a riboprobe (g), (h). 85% of morphant embryos exhibited disrupted Wnt5b expression in the pharyngeal arches compared to controls. 73% of morphant embryos exhibited reduced sox9a expression in the pharyngeal arches compared to controls. Frontal views (a), (b), antero-lateral views (c), (d), and lateral views (e)-(h) with anterior to the right. Red arrows trace the linear outgrowth of the pterygoid process. Red asterisks indicate clumped pterygoid process cells. Red arrowheads indicate the ethmoid. Red brackets indicate the first pharyngeal arch precartilage condensations. E = eye, Eth = ethmoid, Ht = heart tube, Ptp = pterygoid process. Size bars = 100 ยตm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
sox9a AB control Long-pec ethmoid cartilage ISH
Long-pec pharyngeal arch 1 ISH
AB + MO2-wnt9b standard conditions Long-pec ethmoid cartilage ISH
Long-pec pharyngeal arch 1 ISH
wnt5b AB control Long-pec ethmoid cartilage ISH
Long-pec heart ISH
Long-pec palatoquadrate cartilage ISH
Long-pec pharyngeal arch ISH
AB + MO2-wnt9b standard conditions Long-pec ethmoid cartilage ISH
Long-pec heart ISH
Long-pec palatoquadrate cartilage ISH
Long-pec pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-wnt9b standard conditions Long-pec ethmoid cartilage sox9a expression decreased amount, abnormal
Long-pec ethmoid cartilage wnt5b expression decreased amount, abnormal
Long-pec palatoquadrate cartilage wnt5b expression decreased amount, abnormal
Long-pec pharyngeal arch 1 sox9a expression decreased amount, abnormal
Long-pec pharyngeal arch wnt5b expression decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. of Molec. Bio.