ZFIN ID: ZDB-FIG-151112-21
Jackson et al., 2015 - Zebrafish Wnt9b Patterns the First Pharyngeal Arch into D-I-V Domains and Promotes Anterior-Medial Outgrowth. American Journal of Molecular Biology   5(3):57-83 Full text @ Am. J. of Molec. Bio.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5

Fig. 1 Gross morphological phenotypes of 5dpf zebrafish after Wnt9b knockdown. 5dpf live (A)-(D) and Alcian blue stained (E)-(L) embryos, anterior to left, lateral view (A)-(H) and ventral view (I)-(L). Control embryos, injected with the five base mismatched splice blocking morpholino (SB-MM) are shown within the left most column (A), (E), (I) and are indistinguishable from uninjected controls. Splice blocking morpholino (SB-MO) injected embryos were scored as Mild (B), (F), (J), Moderate (C), (G), (K) or Severe (D), (H), (L) using an objective scoring method (see Supplemental Figure 1. for details) and are shown in subsequent columns. (M) Quantification of the proportion of embryos within each category. Translation blocking (TB-MO), splice blocking (SB-MO) and mismatch control (SB-MM) injected embryos were categorically scored at 5dpf. Injection dose is indicated in ng, and the total number (n) of embryos scored is listed. Note that 5.3 ng TB-MO and 2.6 ng SB-MO produce comparable proportions of affected embryos. Error bars are standard error of means of three biological replicates. Size bars in (A)-(L) are 250 um.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-wnt9b standard conditions Day 5 ceratobranchial cartilage decreased size, abnormal
Day 5 ceratohyal cartilage inverted, abnormal
Day 5 cranium anterior region decreased length, abnormal
Day 5 head anterior region flattened, abnormal
Day 5 mandibular arch skeleton malformed, abnormal
Day 5 Meckel's cartilage decreased size, abnormal
Day 5 Meckel's cartilage malformed, abnormal
Day 5 palatoquadrate cartilage fused with Meckel's cartilage, abnormal
Day 5 whole organism has fewer parts of type ceratobranchial cartilage, abnormal
Day 5 whole organism lacks all parts of type swim bladder, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. of Molec. Bio.