ZFIN ID: ZDB-FIG-151112-23
Jackson et al., 2015 - Zebrafish Wnt9b Patterns the First Pharyngeal Arch into D-I-V Domains and Promotes Anterior-Medial Outgrowth. American Journal of Molecular Biology   5(3):57-83 Full text @ Am. J. of Molec. Bio.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 4 Wnt9b morphants exhibit moderate reduction of edn1 expression and the expected expansion in jag1b expression in the pharyngeal arches. (a)-(d) Anterior to right, top row = dorsal view, bottom row = lateral view. MM control (n = 8) (a), (c) and morphant (n = 8) (b, d) embryos were aged to 24hpf and stained with edn1 antisense riboprobe. 63% of morphant embryos exhibited reduced edn1 expression in the pharyngeal arches compared to controls. (e)-(f) Top row = lateral view, bottom row = anterior view. jag1b expression in 48hpf aged MM control (n = 14) (e), (g) and morphant (n = 10) (f)-(h) embryos. 100% of morphant embryos exhibited expanded jag1b expression in the pharyngeal arches compared to controls. White arrowheads indicate PA1. Red brackets indicate PA1. Size bars = 100 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
edn1 AB control Prim-5 pharyngeal arch ISH
AB + MO2-wnt9b standard conditions Prim-5 pharyngeal arch ISH
jag1b AB control Long-pec pharyngeal arch ISH
AB + MO2-wnt9b standard conditions Long-pec pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-wnt9b standard conditions Prim-5 pharyngeal arch edn1 expression decreased amount, abnormal
Long-pec pharyngeal arch jag1b expression increased distribution, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. of Molec. Bio.