ZFIN ID: ZDB-FIG-141023-13
Alexander et al., 2014 - Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton. PLoS Genetics   10:e1004479 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-15

Fig. 4 Requirements for Wnt signaling in dorsal-ventral arch patterning.

(A–P) Whole mount ISH for genes involved in dorsal-ventral (D-V) patterning in control (A, D, G, J), dkk1+ embryos heat shocked at 20–22 hpf (B, E, H, K), and dntcf3+ embryos heat shocked at 22–24 hpf (C, F, I, L), lateral views, anterior to the left. hand2 (B, C), dlx3b (E, F), and dlx6a (H, I) expression is reduced, while jag1b (K, L) expression expands ventrally (arrowheads) particularly in dntcf3+ embryos, compared with controls. (M–P) dlx2a expression in control (M,O), dntcf3+ (N), and dkk1+ (P) embryos. The dorsal boundary of dlx2a expression (arrowheads) appears to shift ventrally in some cases, (N,P) compared to the control (M,O). (Q) Histogram quantifying percentages of dkk1+ and dntcf3+ embryos with defects in D-V patterning. Scale bar: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a WT control Prim-15 pharyngeal arch neural crest ISH
w26Tg/+ heat shock Prim-15 pharyngeal arch neural crest ISH
w32Tg/w32Tg heat shock Prim-15 pharyngeal arch neural crest ISH
dlx3b WT control Prim-15 pharyngeal arch ISH
w26Tg/+ heat shock Prim-15 pharyngeal arch ISH
w32Tg/w32Tg heat shock Prim-15 pharyngeal arch ISH
dlx6a WT control Prim-15 pharyngeal arch ISH
w26Tg/+ heat shock Prim-15 pharyngeal arch ISH
w32Tg/w32Tg heat shock Prim-15 pharyngeal arch ISH
hand2 WT control Prim-15 pharyngeal arch ventral region ISH
w26Tg/+ heat shock Prim-15 pharyngeal arch ventral region ISH
w32Tg/w32Tg heat shock Prim-15 pharyngeal arch ventral region ISH
jag1b WT control Prim-15 pharyngeal arch dorsal-most region ISH
w26Tg/+ heat shock Prim-15 pharyngeal arch ISH
w32Tg/w32Tg heat shock Prim-15 pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.