ZFIN ID: ZDB-FIG-141023-24
Alexander et al., 2014 - Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton. PLoS Genetics   10:e1004479 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: High-pec to Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. S11

Defects in endoderm and oral ectoderm in dkk1+ embryos (A–L, N–Q) Whole mount ISH, anterior to the left. (A–H) Dorsal and lateral views showing nkx2.3 expression in pharyngeal pouch endoderm at 50 hpf in controls (A, C, E, G), dntcf3+ (B, F), and dkk1+ (D, H) embryos. Normal pouches are numbered. Asterisks and black lines indicate defective pouches. (I–L, N–Q) Ventral views showing pitx2ca expression in control (I–L) and dkk1+ (N–Q) embryos. (M, R) Transverse sections of the mouth showing pitx2ca expression in control (M) and dkk1+ (R) embryos at 51 hpf. Arrowheads indicate expression in oral ectoderm. Dotted lines in K, L, P and Q indicate the mouth opening. White arrowhead indicates a ventral midline fold in the mouth. Scale bars: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
nkx2.3 WT control Long-pec pharyngeal pouch 1 ISH
Long-pec pharyngeal pouch 2 ISH
Long-pec pharyngeal pouch 3 ISH
Long-pec pharyngeal pouch 4 ISH
Long-pec pharyngeal pouch 5 ISH
w26Tg/+ heat shock Long-pec pharyngeal pouch ISH
Long-pec pharyngeal pouch 1 ISH
Long-pec pharyngeal pouch 2 ISH
w32Tg/w32Tg heat shock Long-pec pharyngeal pouch ISH
Long-pec pharyngeal pouch 1 ISH
Long-pec pharyngeal pouch 2 ISH
pitx2 WT control High-pec brain ISH
High-pec stomodeum ISH
Long-pec brain ISH
Long-pec oral ectoderm ISH
Pec-fin to Protruding-mouth oral ectoderm ISH
w32Tg/w32Tg heat shock High-pec brain ISH
High-pec Not Detected stomodeum ISH
Long-pec brain ISH
Long-pec oral ectoderm ISH
Pec-fin to Protruding-mouth oral ectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w26Tg/+ heat shock Long-pec pharyngeal pouch morphology, abnormal
w32Tg/w32Tg heat shock Long-pec pharyngeal pouch morphology, abnormal
Pec-fin - Protruding-mouth mouth morphology, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.