ZFIN Figure: Alexander et al., 2014, Fig. S11

ZFIN ID: ZDB-FIG-141023-24

ZFIN ID: ZDB-FIG-141023-24

Alexander et al., 2014 - Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton. PLoS Genetics 10:e1004479 Full text @ PLoS Genet.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	nkx2.3 pitx2
Fish:	w26Tg/+ w32Tg/w32Tg WT
Condition:	heat shock
Anatomical Terms:	brain oral ectoderm pharyngeal pouch pharyngeal pouch 1 pharyngeal pouch 2 pharyngeal pouch 3 pharyngeal pouch 4 pharyngeal pouch 5 stomodeum
Stage Range:	High-pec to Protruding-mouth

PHENOTYPE:

Fish:	w26Tg/+ w32Tg/w32Tg
Condition:	heat shock
Observed In:	mouth pharyngeal pouch
Stage Range:	Long-pec to Protruding-mouth

Fig. S11

Defects in endoderm and oral ectoderm in dkk1+ embryos (A–L, N–Q) Whole mount ISH, anterior to the left. (A–H) Dorsal and lateral views showing nkx2.3 expression in pharyngeal pouch endoderm at 50 hpf in controls (A, C, E, G), dntcf3+ (B, F), and dkk1+ (D, H) embryos. Normal pouches are numbered. Asterisks and black lines indicate defective pouches. (I–L, N–Q) Ventral views showing pitx2ca expression in control (I–L) and dkk1+ (N–Q) embryos. (M, R) Transverse sections of the mouth showing pitx2ca expression in control (M) and dkk1+ (R) embryos at 51 hpf. Arrowheads indicate expression in oral ectoderm. Dotted lines in K, L, P and Q indicate the mouth opening. White arrowhead indicates a ventral midline fold in the mouth. Scale bars: 100 µm.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
nkx2.3	WT	control	Long-pec		pharyngeal pouch 1	ISH
			Long-pec		pharyngeal pouch 2	ISH
			Long-pec		pharyngeal pouch 3	ISH
			Long-pec		pharyngeal pouch 4	ISH
			Long-pec		pharyngeal pouch 5	ISH
	w26Tg/+	heat shock	Long-pec		pharyngeal pouch	ISH
			Long-pec		pharyngeal pouch 1	ISH
			Long-pec		pharyngeal pouch 2	ISH
	w32Tg/w32Tg	heat shock	Long-pec		pharyngeal pouch	ISH
			Long-pec		pharyngeal pouch 1	ISH
			Long-pec		pharyngeal pouch 2	ISH
pitx2	WT	control	High-pec		brain	ISH
			High-pec		stomodeum	ISH
			Long-pec		brain	ISH
			Long-pec		oral ectoderm	ISH
			Pec-fin to Protruding-mouth		oral ectoderm	ISH
	w32Tg/w32Tg	heat shock	High-pec		brain	ISH
			High-pec	Not Detected	stomodeum	ISH
			Long-pec		brain	ISH
			Long-pec		oral ectoderm	ISH
			Pec-fin to Protruding-mouth		oral ectoderm	ISH

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
w26Tg/+	heat shock	Long-pec	pharyngeal pouch morphology, abnormal
w32Tg/w32Tg	heat shock	Long-pec	pharyngeal pouch morphology, abnormal
		Pec-fin - Protruding-mouth	mouth morphology, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.