ZFIN ID: ZDB-FIG-141023-24 |
Genes: | |
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Fish: | |
Condition: | |
Anatomical Terms: | |
Stage Range: | High-pec to Protruding-mouth |
Fish: | |
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Condition: | |
Observed In: | |
Stage Range: | Long-pec to Protruding-mouth |
Fig. S11 Defects in endoderm and oral ectoderm in dkk1+ embryos (A–L, N–Q) Whole mount ISH, anterior to the left. (A–H) Dorsal and lateral views showing nkx2.3 expression in pharyngeal pouch endoderm at 50 hpf in controls (A, C, E, G), dntcf3+ (B, F), and dkk1+ (D, H) embryos. Normal pouches are numbered. Asterisks and black lines indicate defective pouches. (I–L, N–Q) Ventral views showing pitx2ca expression in control (I–L) and dkk1+ (N–Q) embryos. (M, R) Transverse sections of the mouth showing pitx2ca expression in control (M) and dkk1+ (R) embryos at 51 hpf. Arrowheads indicate expression in oral ectoderm. Dotted lines in K, L, P and Q indicate the mouth opening. White arrowhead indicates a ventral midline fold in the mouth. Scale bars: 100 µm. |
Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
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nkx2.3 | WT | control | Long-pec | pharyngeal pouch 1 | ISH | ||
Long-pec | pharyngeal pouch 2 | ISH | |||||
Long-pec | pharyngeal pouch 3 | ISH | |||||
Long-pec | pharyngeal pouch 4 | ISH | |||||
Long-pec | pharyngeal pouch 5 | ISH | |||||
w26Tg/+ | heat shock | Long-pec | pharyngeal pouch | ISH | |||
Long-pec | pharyngeal pouch 1 | ISH | |||||
Long-pec | pharyngeal pouch 2 | ISH | |||||
w32Tg/w32Tg | heat shock | Long-pec | pharyngeal pouch | ISH | |||
Long-pec | pharyngeal pouch 1 | ISH | |||||
Long-pec | pharyngeal pouch 2 | ISH | |||||
pitx2 | WT | control | High-pec | brain | ISH | ||
High-pec | stomodeum | ISH | |||||
Long-pec | brain | ISH | |||||
Long-pec | oral ectoderm | ISH | |||||
Pec-fin to Protruding-mouth | oral ectoderm | ISH | |||||
w32Tg/w32Tg | heat shock | High-pec | brain | ISH | |||
High-pec | Not Detected | stomodeum | ISH | ||||
Long-pec | brain | ISH | |||||
Long-pec | oral ectoderm | ISH | |||||
Pec-fin to Protruding-mouth | oral ectoderm | ISH |
Acknowledgments: | This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet. |
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