ZFIN ID: ZDB-FIG-141023-12
Alexander et al., 2014 - Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton. PLoS Genetics   10:e1004479 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to High-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-5 to High-pec

Fig. 3 Requirements for Wnt signaling in pharyngeal arch cell proliferation.

(A–L) Whole mount ISH for pcna in embryos fixed at 3–22 hours post-heat shock (hphs), lateral views, anterior to the left (all heat shocked at 22 hpf). (A–D) pcna is expressed throughout arches, brain and eyes. (E–H) dkk1+ embryos have reduced pcna expression at 3 hphs (E), severe reductions at 6 hphs (F) and virtually no arch expression at 8 hphs (G), before expression returns at 22 hphs (H). (I–L) dntcf3+ embryos show reduced pcna expression at 3 hphs (I), and virtually no expression at 6 hphs (J, K) before expression rebounds between 8–22 hphs (L). (M) Histogram quantifying percentages of dkk1+ and dntcf3+ embryos with moderate versus severe reductions in pcna expression. Scale bar: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pcna WT control Prim-5 brain ISH
Prim-5 eye ISH
Prim-5 pharyngeal arch ISH
Prim-15 brain ISH
Prim-15 eye ISH
Prim-15 pharyngeal arch ISH
High-pec brain ISH
High-pec eye ISH
High-pec pharyngeal arch ISH
w26Tg/+ heat shock Prim-5 brain ISH
Prim-5 eye ISH
Prim-5 pharyngeal arch ISH
Prim-15 brain ISH
Prim-15 eye ISH
Prim-15 pharyngeal arch ISH
High-pec brain ISH
High-pec eye ISH
High-pec pharyngeal arch ISH
w32Tg/w32Tg heat shock Prim-5 brain ISH
Prim-5 eye ISH
Prim-5 pharyngeal arch ISH
Prim-15 brain ISH
Prim-15 eye ISH
Prim-15 pharyngeal arch ISH
High-pec brain ISH
High-pec eye ISH
High-pec pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w26Tg/+ heat shock Prim-5 pharyngeal arch cell population proliferation decreased process quality, abnormal
Prim-15 pharyngeal arch cell population proliferation decreased process quality, abnormal
High-pec pharyngeal arch cell population proliferation decreased process quality, abnormal
w32Tg/w32Tg heat shock Prim-5 pharyngeal arch cell population proliferation decreased process quality, abnormal
Prim-15 pharyngeal arch cell population proliferation decreased process quality, abnormal
High-pec pharyngeal arch cell population proliferation decreased process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.