ZFIN ID: ZDB-FIG-141023-10 |
Genes: | |
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Fish: | |
Condition: | |
Anatomical Terms: | |
Stage: | Prim-5 |
Fig. 1 Wnt signaling in the pharyngeal arches. (A–H) In situ hybridization (ISH) and (I) quantitative, real-time PCR (qPCR) analysis of Wnt target gene expression; (A, C, E–H) lateral views, anterior to the left; (B, D) transverse sections through arch 2. (A, B) mycn mRNA is ventrally restricted (black arrowheads) in wild type (WT) embryos. Arches 1 and 2 are outlined by dotted lines. (C, D) GFP mRNA is ventrally restricted (arrowheads) in Tg(7xTCF-Xla.Siam:GFP)ia4 transgenics. (B, D) Both mycn (B) and 7xTCF;GFP (D) are expressed in ventral nc cells and pharyngeal endoderm, and excluded from pharyngeal ectoderm. (E–H) mycn and axin2 expression in controls (E, G), and dntcf3+ embryos at 26 hpf (F, H; heat shocked at 22 hpf). (I) qPCR analysis of axin2, lef1 and mycn expression in dntcf3+ embryos, normalized to nontransgenic, heat-shocked controls, with ef1alpha as an internal control. * P<0.05, ** P<0.001. Abbreviations: e, eye; D, dorsal arch; end, pharyngeal endoderm; hb, hindbrain; I, intermediate arch; mhb, mid-hindbrain boundary; nc, neural crest; pe, pharyngeal ectoderm; V, ventral arch. Scale bars: 100 µm. |
Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
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axin2 | WT | control | Prim-5 | eye | ISH | ||
Prim-5 | hindbrain | ISH | |||||
Prim-5 | midbrain | ISH | |||||
w26Tg/+ | heat shock | Prim-5 | eye | ISH | |||
Prim-5 | hindbrain | ISH | |||||
Prim-5 | midbrain | ISH | |||||
GFP | ia4Tg | standard conditions | Prim-5 | pharyngeal arch neural crest | ISH | ||
Prim-5 | Not Detected | pharyngeal arch pharyngeal ectoderm | ISH | ||||
Prim-5 | pharyngeal arch pharyngeal endoderm | ISH | |||||
Prim-5 | pharyngeal arch ventral region | ISH | |||||
mycn | WT | control | Prim-5 | brain | ISH | ||
Prim-5 | eye | ISH | |||||
Prim-5 | pharyngeal arch ventral region | ISH | |||||
WT | standard conditions | Prim-5 | pharyngeal arch neural crest | ISH | |||
Prim-5 | pharyngeal arch ventral region | ISH | |||||
w26Tg/+ | heat shock | Prim-5 | brain | ISH | |||
Prim-5 | eye | ISH | |||||
Prim-5 | pharyngeal arch ventral region | ISH |
Acknowledgments: | This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet. |
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