ZFIN ID: ZDB-FIG-141023-10
Alexander et al., 2014 - Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton. PLoS Genetics   10:e1004479 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-5

Fig. 1 Wnt signaling in the pharyngeal arches.

(A–H) In situ hybridization (ISH) and (I) quantitative, real-time PCR (qPCR) analysis of Wnt target gene expression; (A, C, E–H) lateral views, anterior to the left; (B, D) transverse sections through arch 2. (A, B) mycn mRNA is ventrally restricted (black arrowheads) in wild type (WT) embryos. Arches 1 and 2 are outlined by dotted lines. (C, D) GFP mRNA is ventrally restricted (arrowheads) in Tg(7xTCF-Xla.Siam:GFP)ia4 transgenics. (B, D) Both mycn (B) and 7xTCF;GFP (D) are expressed in ventral nc cells and pharyngeal endoderm, and excluded from pharyngeal ectoderm. (E–H) mycn and axin2 expression in controls (E, G), and dntcf3+ embryos at 26 hpf (F, H; heat shocked at 22 hpf). (I) qPCR analysis of axin2, lef1 and mycn expression in dntcf3+ embryos, normalized to nontransgenic, heat-shocked controls, with ef1alpha as an internal control. * P<0.05, ** P<0.001. Abbreviations: e, eye; D, dorsal arch; end, pharyngeal endoderm; hb, hindbrain; I, intermediate arch; mhb, mid-hindbrain boundary; nc, neural crest; pe, pharyngeal ectoderm; V, ventral arch. Scale bars: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
axin2 WT control Prim-5 eye ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ISH
w26Tg/+ heat shock Prim-5 eye ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ISH
GFP ia4Tg standard conditions Prim-5 pharyngeal arch neural crest ISH
Prim-5 Not Detected pharyngeal arch pharyngeal ectoderm ISH
Prim-5 pharyngeal arch pharyngeal endoderm ISH
Prim-5 pharyngeal arch ventral region ISH
mycn WT control Prim-5 brain ISH
Prim-5 eye ISH
Prim-5 pharyngeal arch ventral region ISH
WT standard conditions Prim-5 pharyngeal arch neural crest ISH
Prim-5 pharyngeal arch ventral region ISH
w26Tg/+ heat shock Prim-5 brain ISH
Prim-5 eye ISH
Prim-5 pharyngeal arch ventral region ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.