ZFIN ID: ZDB-FIG-141023-11
Alexander et al., 2014 - Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton. PLoS Genetics   10:e1004479 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 4

Fig. 2 Requirements for Wnt signaling in craniofacial cartilage development.

(A–C, F, G) Alcian blue stained cartilage at 96 hpf, dissected and flat mounted, ventral views, anterior to the left. (A) Wild-type control, (B) dkk1+/ heterozygote, (C) dkk1+/+ homozygote, (F) dntcf3+/ heterozygote, (G) dntcf3+/+ homozygote, heat shocked as described (see Results). Arrowheads indicate clefting of Meckel′s cartilage (Mc). (D) Histogram quantifying the frequency of Mc defects in dkk1+ embryos heat shocked at different stages from 16–36 hpf. (E) Histogram quantifying average Mc length, p<0.01. (H) Histogram quantifying the frequency of cartilage defects in arches 1 and 2 in dntcf3+ embryos heat shocked at different stages from 16–30 hpf. Abbreviations: Ch, ceratohyal; Hm, hyomandibular; Mc, Meckels; Pq, palatoquadrate; Sy, symplectic. Scale bar: 100 µm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w26Tg/+ heat shock Day 4 ceratohyal cartilage absent, abnormal
Day 4 ceratohyal cartilage decreased size, abnormal
Day 4 Meckel's cartilage decreased size, abnormal
Day 4 Meckel's cartilage fused with palatoquadrate cartilage, abnormal
w26Tg/w26Tg heat shock Day 4 ceratohyal cartilage absent, abnormal
Day 4 ceratohyal cartilage decreased size, abnormal
Day 4 Meckel's cartilage decreased size, abnormal
Day 4 Meckel's cartilage fused with palatoquadrate cartilage, abnormal
w32Tg/+ heat shock Day 4 Meckel's cartilage decreased size, abnormal
Day 4 Meckel's cartilage split, abnormal
Day 4 pharyngeal arch cartilage decreased size, abnormal
w32Tg/w32Tg heat shock Day 4 ceratohyal cartilage decreased size, abnormal
Day 4 hyomandibula decreased size, abnormal
Day 4 Meckel's cartilage decreased size, abnormal
Day 4 palatoquadrate cartilage decreased size, abnormal
Day 4 pharyngeal arch cartilage decreased size, abnormal
Day 4 symplectic decreased size, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.