ZFIN ID: ZDB-FIG-141023-15
Alexander et al., 2014 - Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton. PLoS Genetics   10:e1004479 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15

Fig. 7 Bmp and Edn1 restore ventral-intermediate gene expression, but not hand2, in dntcf3+ embryos.

(A–P) Whole mount ISH for ventral-intermediate patterning genes in heat shocked controls (A, E, I, M), dntcf3+ embryos (B, F, J, N), and dntcf3+ embryos with either 25 ng BMP4/7 (C, G, K, O), or 5 ng EDN1 (D, H, L, P), lateral views, anterior to the left. Asterisks in G and K indicate cases in which rescue occurred in arch 1 but not arch 2. (Q) Histogram quantifying the number of embryos with reductions in arch expression for each gene. Quantification obtained by counting number of embryos with rescue of gene expression in three independent experiments (minimum of 10 embryos per protein injection experiment). Statistical method used is a paired student t-test. * P<0.05, ** P<0.001. Scale bar: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx3b WT control Prim-5 to Prim-15 pharyngeal arch ISH
w26Tg/+ heat shock Prim-5 to Prim-15 pharyngeal arch ISH
dlx5a WT control Prim-5 to Prim-15 pharyngeal arch ISH
w26Tg/+ heat shock Prim-5 to Prim-15 pharyngeal arch ISH
hand2 WT control Prim-5 to Prim-15 pharyngeal arch ventral region ISH
w26Tg/+ heat shock Prim-5 to Prim-15 pharyngeal arch ISH
msx1a WT control Prim-5 to Prim-15 pharyngeal arch ISH
Prim-5 to Prim-15 pharyngeal arch ventral region ISH
w26Tg/+ heat shock Prim-5 to Prim-15 pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.