ZFIN ID: ZDB-FIG-111025-25
Madelaine et al., 2011 - Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis. Development (Cambridge, England)   138(21):4753-4762 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Prim-25

Fig. 7

In neurog1 mutant zebrafish embryos, residual early-born olfactory neurons fail to express certain EON markers. (A-I) Confocal sections of in situ hybridisation and immunolabelling against robo2 and GFP from the Tg(8.4neurog1:GFP) transgene at 24 hpf in wild type (A-C), neurog1 mutant embryos (D-F) and neurog1 mutant embryos in which Neurod4 has been mis-expressed (G-I). Whereas early-born olfactory neurons in wild-type embryos robustly express robo2, expression is absent in neurog1 mutant embryos; mis-expression of Neurod4 partially rescues robo2 expression. (J-O) Confocal sections of in situ hybridisation and immunolabelling against robo2 and GFP from the Tg(8.4neurog1:GFP) transgene in wild-type (J-L) and neurog1 mutant (M-O) embryos at 36 hpf. The expression of robo2 never recovers in the few early-born olfactory neurons that develop in neurog1 mutant embryos. Scale bars: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 olfactory placode pioneer neuron IHC
Prim-25 peripheral olfactory organ IHC
sb1Tg standard conditions Prim-5 olfactory placode pioneer neuron IHC
Prim-25 peripheral olfactory organ IHC
robo2 neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 Not Detected olfactory placode pioneer neuron ISH
Prim-25 Not Detected peripheral olfactory organ olfactory receptor cell ISH
sb1Tg standard conditions Prim-5 olfactory placode pioneer neuron ISH
Prim-25 peripheral olfactory organ sensory neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 axonogenesis disrupted, abnormal
Prim-25 axonogenesis disrupted, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development