ZFIN ID: ZDB-FIG-111025-19
Madelaine et al., 2011 - Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis. Development (Cambridge, England)   138(21):4753-4762 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 1

Tg(8.4neurog1:gfp) is a marker of zebrafish early-born olfactory neurons. (A-F) Single confocal sections of olfactory placodes showing co-expression of HuC/D (A-C) or the previously described pioneer neuron marker zns-2 (D-F) with GFP from the Tg(8.4neurog1:GFP) transgene at 24 hpf. (G-L) Single confocal sections of olfactory placodes showing immunolabelling of HuC/D (G-I) or in situ hybridisation and immunolabelling of omp (J-L) and GFP from the Tg(8.4neurog1:GFP) transgene at 48 hpf. Whereas all GFP-positive cells in the placode are HuC/D- and zns-2-positive at 24 hpf, at 48 hpf HuC/D+/GFP–cells are present; little, if any, overlap is detected between the expression of omp transcripts and GFP at 48 hpf. (M,N) Schematics of olfactory placodes at 24 hpf (M) or 48 hpf (N). Early-born olfactory neurons are shown in green and olfactory sensory neurons in red. A, anterior; L, lateral; M, medial; P, posterior; T, telencephalon. Scale bars: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP sb1Tg standard conditions Prim-5 olfactory placode IHC
Long-pec peripheral olfactory organ IHC
ompb sb1Tg standard conditions Long-pec peripheral olfactory organ olfactory receptor cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC sb1Tg standard conditions Prim-5 olfactory placode
IHC Long-pec peripheral olfactory organ
zns-2 IHC sb1Tg standard conditions Prim-5 olfactory placode pioneer neuron
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development