ZFIN ID: ZDB-FIG-111025-22
Madelaine et al., 2011 - Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis. Development (Cambridge, England)   138(21):4753-4762 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 4

Expression of neurod4 in the zebrafish olfactory anlage is dependent on neurog1 at early but not late stages. (A-D,H-K) Whole-mount in situ hybridisation against neurod4 in wild-type (A-D) and neurog1 mutant (H-K) embryos at 11 (A,H), 13 (B,I), 15 (C,J) and 17 (D,K) hpf. Although neurod4 expression is absent in mutant embryos up to 15 hpf, expression recovers to wild-type levels at 17 hpf. Embryos are viewed dorsally with anterior down; black arrowheads indicate the mixed telencephalo-olfactory expression domain. (E-G,L-N) Single confocal sections of in situ hybridisation and immunolabelling against neurod4 and GFP from the Tg(8.4neurog1:GFP) transgene in the olfactory placode of wild-type (E-G) or neurog1 mutant (L-N) embryos at 19 (E,L), 22 (F,M) and 24 (G,N) hpf. At 24 hpf, neurod4 is restricted to cells of the olfactory placode immediately adjacent to the telencephalon and is excluded from Tg(8.4neurog1:gfp)-positive early-born olfactory neurons. White arrowheads indicate double-labelled cells. Dashed white line indicates the boundary of the telencephalon and the olfactory placode. Scale bars: in A, 100 μM for A-D; in H, 100 μM for H-K; in E-G,L-N, 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions 20-25 somites olfactory placode IHC
26+ somites olfactory placode IHC
Prim-5 olfactory placode IHC
sb1Tg standard conditions 20-25 somites olfactory placode IHC
26+ somites olfactory placode IHC
Prim-5 olfactory placode IHC
neurod4 neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions 1-4 somites Not Detected olfactory field ISH
5-9 somites Not Detected olfactory field ISH
10-13 somites Not Detected olfactory placode ISH
14-19 somites olfactory placode ISH
14-19 somites telencephalon ISH
20-25 somites olfactory placode ISH
20-25 somites telencephalon ISH
26+ somites olfactory placode ISH
26+ somites Not Detected olfactory placode pioneer neuron ISH
26+ somites telencephalon ISH
Prim-5 olfactory placode ISH
Prim-5 Not Detected olfactory placode pioneer neuron ISH
Prim-5 telencephalon ISH
sb1Tg standard conditions 1-4 somites olfactory field ISH
5-9 somites olfactory field ISH
10-13 somites olfactory field ISH
10-13 somites telencephalon ISH
14-19 somites olfactory placode ISH
14-19 somites telencephalon ISH
20-25 somites olfactory placode ISH
20-25 somites telencephalon ISH
26+ somites olfactory placode ISH
26+ somites telencephalon ISH
Prim-5 olfactory placode ISH
Prim-5 Not Detected olfactory placode pioneer neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions 20-25 somites olfactory placode development delayed, abnormal
26+ somites olfactory placode development delayed, abnormal
Prim-5 olfactory bulb development process quality, normal
Acknowledgments:
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