ZFIN ID: ZDB-FIG-111025-20
Madelaine et al., 2011 - Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis. Development (Cambridge, England)   138(21):4753-4762 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 2

Zebrafish early-born olfactory neuron development is delayed in the absence of neurog1 function. (A-D) Confocal projections of wild-type (A) or neurog1 mutant (B-D) olfactory placodes at 24 hpf labelled for HuC/D and GFP from the Tg(8.4neurog1:GFP) transgene; mutant embryos show a variable but reduced number (shown as percentage of wild type) of early-born olfactory neurons. (E,F) Confocal projections of wild-type (E) or neurog1 mutant (F) olfactory placodes at 22 hpf labelled for HuC/D; a reduced number of HuC/D-positive neurons is detected in the olfactory placode of mutant embryos at this stage. (G,H) Confocal projections of wild-type (G) or neurog1 mutant (H) olfactory placodes at 19 hpf labelled for HuC/D; a complete absence of HuC/D neurons is apparent in the olfactory anlagen in neurog1 mutant embryos. Dashed white line indicates the boundary of the telencephalon and the olfactory placode; arrowheads indicate HuC/D expression in olfactory placode. Scale bars: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 olfactory placode IHC
sb1Tg standard conditions Prim-5 olfactory placode IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions 20-25 somites olfactory placode pioneer neuron
IHC 26+ somites olfactory placode pioneer neuron
IHC Prim-5 olfactory placode pioneer neuron
IHC sb1Tg standard conditions 20-25 somites olfactory placode pioneer neuron
IHC 26+ somites olfactory placode pioneer neuron
IHC Prim-5 olfactory placode pioneer neuron
Phenotype Details
Fish Conditions Stage Phenotype
neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions 20-25 somites olfactory placode development delayed, abnormal
20-25 somites olfactory placode pioneer neuron absent, abnormal
26+ somites axon extension heterochronic, abnormal
26+ somites olfactory placode development delayed, abnormal
26+ somites olfactory placode pioneer neuron decreased amount, abnormal
Prim-5 axon extension heterochronic, abnormal
Prim-5 olfactory placode development delayed, abnormal
Prim-5 olfactory placode pioneer neuron decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development