ZFIN ID: ZDB-FIG-111025-24
Madelaine et al., 2011 - Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis. Development (Cambridge, England)   138(21):4753-4762 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 6

Neurod4 and Neurog1 act redundantly during OSN development in zebrafish. (A-D2) Confocal projections of in situ hybridisation and immunolabelling against deltaA and GFP in wild-type (A,A2), neurod4 morphant (B,B2), neurog1 mutant (C,C2) and neurog1/neurod4 double loss-of-function (D,D2) olfactory placodes of Tg(8.4neurog1:GFP) embryos at 24 hpf. Although no difference is apparent in the expression of deltaA in single loss-of-function embryos relative to wild-type siblings, deltaA expression is absent when both neurog1 and neurod4 function is abolished. (E-P) Confocal sections of in situ hybridisation labelling against olfactory marker protein (omp) in wild-type (E,I,M), neurod4 morphant (F,J,N), neurog1 mutant (G,K,O) and neurog1/neurod4 double loss-of-function (H,L,P) olfactory placodes at 24 (E-H), 36 (I-L) and 48 (M-P) hpf. Although omp expression resembles the wild-type pattern in the single loss-of-function context at 48 hpf, there is a delay in the initiation of omp expression in the absence of Neurog1 function. The expression of omp is never detected when both neurog1 and neurod4 function is abolished. Scale bars: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dla neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 olfactory placode sensory neuron ISH
neurog1hi1059Tg/hi1059Tg; sb1Tg + MO1-neurod4 standard conditions Prim-5 Not Detected olfactory placode sensory neuron ISH
sb1Tg standard conditions Prim-5 olfactory placode sensory neuron ISH
sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode sensory neuron ISH
GFP neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 olfactory placode pioneer neuron IHC
neurog1hi1059Tg/hi1059Tg; sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode pioneer neuron IHC
sb1Tg standard conditions Prim-5 olfactory placode pioneer neuron IHC
sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode pioneer neuron IHC
ompb neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 Not Detected olfactory placode sensory neuron ISH
Prim-25 peripheral olfactory organ olfactory receptor cell ISH
Long-pec peripheral olfactory organ olfactory receptor cell ISH
neurog1hi1059Tg/hi1059Tg; sb1Tg + MO1-neurod4 standard conditions Prim-5 Not Detected olfactory placode sensory neuron ISH
Prim-25 Not Detected peripheral olfactory organ olfactory receptor cell ISH
Long-pec Not Detected peripheral olfactory organ olfactory receptor cell ISH
sb1Tg standard conditions Prim-5 olfactory placode sensory neuron ISH
Prim-25 peripheral olfactory organ olfactory receptor cell ISH
Long-pec peripheral olfactory organ olfactory receptor cell ISH
sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode sensory neuron ISH
Prim-25 peripheral olfactory organ olfactory receptor cell ISH
Long-pec peripheral olfactory organ olfactory receptor cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 olfactory placode pioneer neuron decreased amount, abnormal
Prim-25 olfactory placode pioneer neuron decreased amount, abnormal
Long-pec olfactory placode pioneer neuron decreased amount, abnormal
neurog1hi1059Tg/hi1059Tg; sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode pioneer neuron absent, abnormal
Prim-25 olfactory placode pioneer neuron absent, abnormal
Long-pec olfactory placode pioneer neuron absent, abnormal
sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode pioneer neuron decreased amount, abnormal
Prim-25 olfactory placode pioneer neuron decreased amount, abnormal
Long-pec olfactory placode pioneer neuron decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development