ZFIN ID: ZDB-FIG-111025-30
Madelaine et al., 2011 - Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis. Development (Cambridge, England)   138(21):4753-4762 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-5

Fig. S4

insm1b behaves as a proneural target in the developing zebrafish olfactory system. (A-F) Whole-mount in situ hybridisation against insm1b in wild-type (A-C) and neurog1 mutant (D-F) zebrafish embryos at 13 (A,D), 15 (B,E) or 17 (C,F) hpf. Although insm1b expression is absent in embryos up to 15 hpf in the mutant, its expression recovers at 17 hpf. Embryos are viewed dorsally with anterior down (D-F). (G-J) Confocal projections of in situ hybridisation and immunolabelling against insm1b and GFP in the olfactory placodes of wild-type (G), neurod4 morphant (H), neurog1 mutant (I) and neurog1/neurod4 double loss of function (J) embryos in Tg(8.4neurog1:GFP) embryos at 24 hpf. At this stage, insm1b+ cells are lost only in the neurog1/neurod4 double loss of function situation (J) suggesting that these two proneural factors are redundantly required in olfactory sensory neuron progenitors. Placodes are oriented with anterior up and lateral to the right. Scale bars: in A, 100 μM for A-C; in D, 100 μM for D-F; in G, 10 μM for G-J.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
insm1b WT standard conditions 5-9 somites olfactory field ISH
10-13 somites olfactory field ISH
14-19 somites olfactory placode ISH
neurog1hi1059Tg/hi1059Tg standard conditions 5-9 somites Not Detected olfactory field ISH
10-13 somites Not Detected olfactory field ISH
14-19 somites olfactory placode ISH
neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 unspecified ISH
neurog1hi1059Tg/hi1059Tg; sb1Tg + MO1-neurod4 standard conditions Prim-5 unspecified ISH
sb1Tg standard conditions Prim-5 unspecified ISH
sb1Tg + MO1-neurod4 standard conditions Prim-5 unspecified ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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