ZFIN ID: ZDB-FIG-110407-28
Yee et al., 2011 - Transient receptor potential ion channel Trpm7 regulates exocrine pancreatic epithelial proliferation by Mg2+-sensitive Socs3a signaling in development and cancer. Disease models & mechanisms   4(2):240-254 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 4

Antisense inhibition of zebrafish trpm7 expression phenocopies the swd mutation. (A) Bright-field images (right lateral view) and whole-mount in situ hybridization using anti-trypsin riboprobes (viewed in the dorsoventral direction) at 96 hpf. Note the region of pigmented skin (green arrows) and trypsin-expressing exocrine pancreas (red arrows) in the trpm7-ATG-MO-injected larvae as compared with the control. For in situ hybridization, the control-MO-injected embryos were grown in medium supplemented with PTU, which inhibits skin pigmentation, in order to facilitate visualization of the trypsin-expressing exocrine pancreas. This experiment was performed three times, with 25 embryos being injected for each group in each experiment. About 80–90% of larvae in each experiment exhibit the phenotype shown. The residual skin pigmentation is probably due to incomplete knockdown of trpm7 by the MO. (B) Acinar size and morphology revealed by immunohistochemistry using anti-Cpa or anti-cadherin (Cad) antibodies, followed by histological analysis at 96 hpf. Staining with DAPI was used to visualize the nuclei. The histological sections are oriented as indicated: d, dorsal; v, ventral; r, right; l, left. (C) Proportion of exocrine pancreatic epithelia in the S phase of the cell cycle (% BrdU+ nuclei) at 72 hpf. (D) Pancreatic epithelial cell growth (area in μm2 per cell) by morphometric analysis at 96 hpf. Each value represents the mean + s.d. *P<0.05 indicates statistically significant difference.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
prss1 AB control Day 4 exocrine pancreas ISH
AB + MO2-trpm7 standard conditions Day 4 exocrine pancreas ISH
swdp75fm/p75fm standard conditions Day 4 exocrine pancreas ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-CPA IHC AB control Day 4 acinar cell
IHC Day 4 exocrine pancreas
IHC AB + MO2-trpm7 standard conditions Day 4 acinar cell
IHC Day 4 exocrine pancreas
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-trpm7 standard conditions Protruding-mouth epithelial cell proliferation decreased occurrence, abnormal
Protruding-mouth mitotic cell cycle disrupted, abnormal
Day 4 acinar cell decreased size, abnormal
Day 4 epithelial cell of pancreas decreased area, abnormal
Day 4 exocrine pancreas hypoplastic, abnormal
Day 4 integument decreased pigmentation, abnormal
swdp75fm/p75fm standard conditions Day 4 exocrine pancreas hypoplastic, abnormal
Day 4 integument decreased pigmentation, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.