ZFIN ID: ZDB-FIG-110407-26
Yee et al., 2011 - Transient receptor potential ion channel Trpm7 regulates exocrine pancreatic epithelial proliferation by Mg2+-sensitive Socs3a signaling in development and cancer. Disease models & mechanisms   4(2):240-254 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1

The zebrafish swdp75fm and swdp82mf mutations cause exocrine pancreatic hypoplasia and reduced skin pigmentation. (A–F) Bright-field images of the swd mutants and wild-type (wt) larvae. Green arrows point to pigmented skin. (G–I) Exocrine pancreas (red arrows) revealed by whole-mount in situ hybridization using anti-trypsin riboprobes. (J,K) Histological transverse sections of exocrine pancreas following immunohistochemistry using anti-Cpa antibodies. Staining with DAPI was used to visualize the nuclei. The histological sections are oriented as indicated: d, dorsal; v, ventral; r, right; l, left. (L,M) Exocrine pancreas (red arrows) revealed by whole-mount in situ hybridization using anti-ptf1a riboprobes. (N,O) Sagittal histological sections of larvae. e.p., exocrine pancreas; i, intestine; l, liver. (G,L) wt larvae were grown in E3 medium supplemented with PTU, which inhibits skin pigmentation, in order to facilitate visualization of the exocrine pancreas expressing trypsin or ptf1a. Note that the larvae shown in A–O were analyzed on 5 dpf. (A–C,G–I,L–O) The larvae are positioned in the same orientation and viewed from the right lateral side. (D–F) The views are in the dorsoventral direction.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
prss1 AB standard conditions Day 5 exocrine pancreas ISH
swdp75fm/p75fm standard conditions Day 5 exocrine pancreas ISH
swdp82mf/p82mf standard conditions Day 5 exocrine pancreas ISH
ptf1a AB standard conditions Day 5 exocrine pancreas ISH
swdp75fm/p75fm standard conditions Day 5 exocrine pancreas ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-CPA IHC AB standard conditions Day 5 acinar cell
IHC Day 5 exocrine pancreas
IHC swdp75fm/p75fm standard conditions Day 5 acinar cell
IHC Day 5 exocrine pancreas
Phenotype Details
Fish Conditions Stage Phenotype
swdp75fm/p75fm standard conditions Day 5 acinar cell decreased size, abnormal
Day 5 acinar cell zymogen granule decreased amount, abnormal
Day 5 exocrine pancreas hypoplastic, abnormal
Day 5 integument decreased pigmentation, abnormal
Day 5 intestine morphology, normal
Day 5 liver morphology, normal
Day 5 pancreatic duct decreased branchiness, abnormal
swdp82mf/p82mf standard conditions Day 5 exocrine pancreas hypoplastic, abnormal
Day 5 integument decreased pigmentation, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.