ZFIN ID: ZDB-FIG-110407-27
Yee et al., 2011 - Transient receptor potential ion channel Trpm7 regulates exocrine pancreatic epithelial proliferation by Mg2+-sensitive Socs3a signaling in development and cancer. Disease models & mechanisms   4(2):240-254 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 3

The swd locus is allelic with the trpm7 gene. (A) Exocrine pancreas of WT, the trpm7j124e1 mutant, the swdp75fm/trpm7j124e1 mutant and the swdp75fm mutant on 5 dpf by immunohistochemistry using anti-Cpa antibodies followed by cross-sectional histological analysis. Staining with DAPI was used to visualize the nuclei. The histological sections are oriented as indicated: d, dorsal; v, ventral; r, right; l, left. (B) Morphometric analysis of exocrine pancreatic epithelial growth (area, in μm2, per cell) in the trpm7j124e1 mutants and WT siblings on 3 and 5 dpf. Each value represents the mean + s.e.m. Statistical analysis was performed using Student’s t-test, with *P<0.05 considered statistically significant. (C) Bright-field images in right lateral view and whole-mount in situ hybridization using anti-trypsin riboprobes. The swdp75fm/+ and trpm7j124e1/+ heterozygotes were crossed with each other, and the progeny larvae were analyzed on 5 dpf. The WT, but not mutant, larvae were grown in medium supplemented with PTU, which inhibits skin pigmentation, in order to facilitate visualization of the exocrine-pancreas-expressing trypsin. Note the regions of skin pigmentation (green arrowheads) and trypsin-expressing exocrine pancreas (red arrows) in the mutants as compared to WT.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
prss1 AB standard conditions Day 5 exocrine pancreas ISH
swdp75fm/p75fm standard conditions Day 5 exocrine pancreas ISH
trpm7j124e1/+; swdp75fm/+ standard conditions Day 5 exocrine pancreas ISH
trpm7j124e1/j124e1 standard conditions Day 5 exocrine pancreas ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-CPA IHC AB standard conditions Day 5 acinar cell
IHC Day 5 exocrine pancreas
IHC swdp75fm/p75fm standard conditions Day 5 acinar cell
IHC Day 5 exocrine pancreas
IHC trpm7j124e1/+; swdp75fm/+ standard conditions Day 5 acinar cell
IHC Day 5 exocrine pancreas
IHC trpm7j124e1/j124e1 standard conditions Day 5 acinar cell
IHC Day 5 exocrine pancreas
Phenotype Details
Fish Conditions Stage Phenotype
swdp75fm/p75fm standard conditions Day 5 acinar cell decreased size, abnormal
Day 5 exocrine pancreas hypoplastic, abnormal
Day 5 integument decreased pigmentation, abnormal
trpm7j124e1/+; swdp75fm/+ standard conditions Day 5 acinar cell decreased size, abnormal
Day 5 exocrine pancreas hypoplastic, abnormal
Day 5 integument decreased pigmentation, abnormal
trpm7j124e1/j124e1 standard conditions Protruding-mouth - Day 5 epithelial cell of pancreas decreased area, abnormal
Day 5 acinar cell decreased size, abnormal
Day 5 exocrine pancreas hypoplastic, abnormal
Day 5 integument decreased pigmentation, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.