PUBLICATION

Transient receptor potential ion channel Trpm7 regulates exocrine pancreatic epithelial proliferation by Mg2+-sensitive Socs3a signaling in development and cancer

Authors
Yee, N.S., Zhou, W., and Liang, I.C.
ID
ZDB-PUB-110103-15
Date
2011
Source
Disease models & mechanisms   4(2): 240-254 (Journal)
Registered Authors
Yee, Nelson S.
Keywords
none
MeSH Terms
  • Alleles
  • Animals
  • Cell Cycle/drug effects
  • Cell Cycle Proteins/genetics
  • Cell Cycle Proteins/metabolism
  • Cell Proliferation/drug effects
  • Epithelial Cells/metabolism
  • Epithelial Cells/pathology*
  • Gene Expression Regulation, Neoplastic/drug effects
  • Genetic Loci
  • Humans
  • Magnesium/pharmacology*
  • Mutation/genetics
  • Oligonucleotides, Antisense/pharmacology
  • Pancreas, Exocrine/abnormalities
  • Pancreas, Exocrine/drug effects
  • Pancreas, Exocrine/embryology
  • Pancreas, Exocrine/pathology*
  • Pancreatic Neoplasms/genetics
  • Pancreatic Neoplasms/pathology*
  • Protein Serine-Threonine Kinases
  • Sequence Homology, Amino Acid
  • Signal Transduction/drug effects
  • Suppressor of Cytokine Signaling Proteins/metabolism*
  • TRPM Cation Channels/genetics
  • TRPM Cation Channels/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
21183474 Full text @ Dis. Model. Mech.
Abstract
Genetic analysis of pancreatic development has provided new insights into the mechanisms underlying the formation of exocrine pancreatic neoplasia. Zebrafish sweetbread (swd) mutants develop hypoplastic acini and dysmorphic ducts in the exocrine pancreas, with impeded progression of cell division cycle and of epithelial growth. Positional cloning and allelic complementation have revealed that the swd mutations affect the transient receptor potential melastatin-subfamily member 7 (trpm7) gene, which encodes a divalent cation-permeable channel with kinase activity. Supplementary Mg(2+) partially rescued the exocrine pancreatic defects of the trpm7 mutants by improving cell-cycle progression and growth and repressing the suppressor of cytokine signaling 3a (socs3a) gene. The role of Socs3a in Trpm7-mediated signaling is supported by the findings that socs3a mRNA level is elevated in the trpm7 mutants, and antisense inhibition of socs3a expression improved their exocrine pancreatic growth. TRPM7 is generally overexpressed in human pancreatic adenocarcinoma. TRPM7-deficient cells are impaired in proliferation and arrested in the G0-G1 phases of the cell division cycle. Supplementary Mg(2+) rescued the proliferative defect of the TRPM7-deficient cells. Results of this study indicate that Trpm7 regulates exocrine pancreatic development via the Mg(2+)-sensitive Socs3a pathway, and suggest that aberrant TRPM7-mediated signaling contributes to pancreatic carcinogenesis.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes