ZFIN ID: ZDB-FIG-110317-12
Osborn et al., 2011 - Cdkn1c drives muscle differentiation through a positive feedback loop with Myod. Developmental Biology   350(2):464-475 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 5-9 somites to 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 5-9 somites to 10-13 somites

Fig. 2 Myod-driven myogenin expression permits slow fibre formation in shha mutant. Myog mRNA or MyHC accumulation in uninjected, myod MO- or myog MO-injected embryos from wild type or shhatbx/+ in-cross. Dorsal flatmounts of 5 ss (A,B) or 10–15 ss (C) embryos. Bar: 100 Μm. (A). Myog mRNA is ablated by myod MO injection into wild type. (B) Myod and myog MOs each delay adaxial myogenesis. (C) Myod and myog, but not myf5, MOs ablate adaxial myogenesis in shhatbx392 mutants (21/79), but not from siblings.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
en2a WT standard conditions 10-13 somites adaxial cell ISH
WT + MO1-myod1 standard conditions 10-13 somites adaxial cell ISH
WT + MO3-myf5 standard conditions 10-13 somites adaxial cell ISH
shhatbx392/tbx392 standard conditions 10-13 somites adaxial cell ISH
shhatbx392/tbx392 + MO1-myod1 standard conditions 10-13 somites Not Detected adaxial cell ISH
shhatbx392/tbx392 + MO3-myf5 standard conditions 10-13 somites adaxial cell ISH
myf6 WT standard conditions 10-13 somites adaxial cell ISH
shhatbx392/tbx392 standard conditions 10-13 somites Not Detected adaxial cell ISH
myod1 WT standard conditions 10-13 somites adaxial cell ISH
10-13 somites somite ISH
shhatbx392/tbx392 standard conditions 10-13 somites Not Detected adaxial cell ISH
10-13 somites somite ISH
myog WT control 5-9 somites adaxial cell ISH
WT + MO1-myod1 standard conditions 5-9 somites Not Detected adaxial cell ISH
prdm1a WT control 5-9 somites adaxial cell ISH
WT + MO1-myog standard conditions 5-9 somites adaxial cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-A4.1025 IHC WT control 5-9 somites adaxial cell
IHC WT + MO1-myod1 standard conditions 5-9 somites Not Detected adaxial cell
IHC WT + MO1-myog standard conditions 5-9 somites adaxial cell
Ab-F59 IHC WT standard conditions 10-13 somites adaxial cell
IHC WT + MO1-myod1 standard conditions 10-13 somites adaxial cell
IHC WT + MO1-myog standard conditions 10-13 somites adaxial cell
IHC WT + MO3-myf5 standard conditions 10-13 somites adaxial cell
IHC shhatbx392/tbx392 standard conditions 10-13 somites adaxial cell
IHC shhatbx392/tbx392 + MO1-myod1 standard conditions 10-13 somites Not Detected adaxial cell
IHC shhatbx392/tbx392 + MO1-myog standard conditions 10-13 somites Not Detected adaxial cell
IHC shhatbx392/tbx392 + MO3-myf5 standard conditions 10-13 somites adaxial cell
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-myod1 standard conditions 5-9 somites skeletal muscle tissue development delayed, abnormal
WT + MO1-myog standard conditions 5-9 somites skeletal muscle tissue development delayed, abnormal
shhatbx392/tbx392 standard conditions 10-13 somites skeletal muscle tissue development delayed, abnormal
shhatbx392/tbx392 + MO1-myod1 standard conditions 10-13 somites skeletal muscle tissue development arrested, abnormal
shhatbx392/tbx392 + MO1-myog standard conditions 10-13 somites skeletal muscle tissue development arrested, abnormal
shhatbx392/tbx392 + MO3-myf5 standard conditions 10-13 somites skeletal muscle tissue development delayed, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.