ZFIN ID: ZDB-FIG-110317-12 |
Osborn et al., 2011
- Cdkn1c drives muscle differentiation through a positive feedback loop with Myod.
Developmental Biology
350(2):464-475
Full text @ Dev. Biol.
EXPRESSION / LABELING:
Genes: | |
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Antibodies: | |
Fish: | |
Knockdown Reagents: | |
Anatomical Terms: | |
Stage Range: | 5-9 somites to 10-13 somites |
PHENOTYPE:
Fish: | |
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Knockdown Reagents: | |
Observed In: | |
Stage Range: | 5-9 somites to 10-13 somites |
Fig. 2 Myod-driven myogenin expression permits slow fibre formation in shha mutant. Myog mRNA or MyHC accumulation in uninjected, myod MO- or myog MO-injected embryos from wild type or shhatbx/+ in-cross. Dorsal flatmounts of 5 ss (A,B) or 10–15 ss (C) embryos. Bar: 100 Μm. (A). Myog mRNA is ablated by myod MO injection into wild type. (B) Myod and myog MOs each delay adaxial myogenesis. (C) Myod and myog, but not myf5, MOs ablate adaxial myogenesis in shhatbx392 mutants (21/79), but not from siblings. |
Gene Expression Details
Antibody Labeling Details
Antibody | Assay | Fish | Conditions | Stage | Qualifier | Anatomy |
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Ab-A4.1025 | IHC | WT | control | 5-9 somites | adaxial cell | |
IHC | WT + MO1-myod1 | standard conditions | 5-9 somites | Not Detected | adaxial cell | |
IHC | WT + MO1-myog | standard conditions | 5-9 somites | adaxial cell | ||
Ab-F59 | IHC | WT | standard conditions | 10-13 somites | adaxial cell | |
IHC | WT + MO1-myod1 | standard conditions | 10-13 somites | adaxial cell | ||
IHC | WT + MO1-myog | standard conditions | 10-13 somites | adaxial cell | ||
IHC | WT + MO3-myf5 | standard conditions | 10-13 somites | adaxial cell | ||
IHC | shhatbx392/tbx392 | standard conditions | 10-13 somites | adaxial cell | ||
IHC | shhatbx392/tbx392 + MO1-myod1 | standard conditions | 10-13 somites | Not Detected | adaxial cell | |
IHC | shhatbx392/tbx392 + MO1-myog | standard conditions | 10-13 somites | Not Detected | adaxial cell | |
IHC | shhatbx392/tbx392 + MO3-myf5 | standard conditions | 10-13 somites | adaxial cell |
Phenotype Details
Acknowledgments: |
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Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol. |
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