ZFIN Figure: Osborn et al., 2011, Fig. 2

ZFIN ID: ZDB-FIG-110317-12

ZFIN ID: ZDB-FIG-110317-12

Osborn et al., 2011 - Cdkn1c drives muscle differentiation through a positive feedback loop with Myod. Developmental Biology 350(2):464-475 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	en2a myf6 myod1 myog prdm1a
Antibodies:	Ab-A4.1025 Ab-F59
Fish:	WT shha^{tbx392/tbx392} WT + MO1-myod1 WT + MO1-myog WT + MO3-myf5 shha^{tbx392/tbx392} + MO1-myod1 shha^{tbx392/tbx392} + MO1-myog shha^{tbx392/tbx392} + MO3-myf5
Knockdown Reagents:	MO1-myod1 MO1-myog MO3-myf5
Anatomical Terms:	adaxial cell somite
Stage Range:	5-9 somites to 10-13 somites

PHENOTYPE:

Fish:	shha^{tbx392/tbx392} WT + MO1-myod1 WT + MO1-myog shha^{tbx392/tbx392} + MO1-myod1 shha^{tbx392/tbx392} + MO1-myog shha^{tbx392/tbx392} + MO3-myf5
Knockdown Reagents:	MO1-myod1 MO1-myog MO3-myf5
Observed In:	skeletal muscle tissue development
Stage Range:	5-9 somites to 10-13 somites

Fig. 2 Myod-driven myogenin expression permits slow fibre formation in shha mutant. Myog mRNA or MyHC accumulation in uninjected, myod MO- or myog MO-injected embryos from wild type or shha^tbx/+ in-cross. Dorsal flatmounts of 5 ss (A,B) or 10–15 ss (C) embryos. Bar: 100 &MU;m. (A). Myog mRNA is ablated by myod MO injection into wild type. (B) Myod and myog MOs each delay adaxial myogenesis. (C) Myod and myog, but not myf5, MOs ablate adaxial myogenesis in shha^tbx392 mutants (21/79), but not from siblings.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
en2a	WT	standard conditions	10-13 somites		adaxial cell	ISH
	WT + MO1-myod1	standard conditions	10-13 somites		adaxial cell	ISH
	WT + MO3-myf5	standard conditions	10-13 somites		adaxial cell	ISH
	shha^{tbx392/tbx392}	standard conditions	10-13 somites		adaxial cell	ISH
	shha^{tbx392/tbx392} + MO1-myod1	standard conditions	10-13 somites	Not Detected	adaxial cell	ISH
	shha^{tbx392/tbx392} + MO3-myf5	standard conditions	10-13 somites		adaxial cell	ISH
myf6	WT	standard conditions	10-13 somites		adaxial cell	ISH
	shha^{tbx392/tbx392}	standard conditions	10-13 somites	Not Detected	adaxial cell	ISH
myod1	WT	standard conditions	10-13 somites		adaxial cell	ISH
			10-13 somites		somite	ISH
	shha^{tbx392/tbx392}	standard conditions	10-13 somites	Not Detected	adaxial cell	ISH
			10-13 somites		somite	ISH
myog	WT	control	5-9 somites		adaxial cell	ISH
	WT + MO1-myod1	standard conditions	5-9 somites	Not Detected	adaxial cell	ISH
prdm1a	WT	control	5-9 somites		adaxial cell	ISH
	WT + MO1-myog	standard conditions	5-9 somites		adaxial cell	ISH

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Qualifier	Anatomy
Ab-A4.1025	IHC	WT	control	5-9 somites		adaxial cell
	IHC	WT + MO1-myod1	standard conditions	5-9 somites	Not Detected	adaxial cell
	IHC	WT + MO1-myog	standard conditions	5-9 somites		adaxial cell
Ab-F59	IHC	WT	standard conditions	10-13 somites		adaxial cell
	IHC	WT + MO1-myod1	standard conditions	10-13 somites		adaxial cell
	IHC	WT + MO1-myog	standard conditions	10-13 somites		adaxial cell
	IHC	WT + MO3-myf5	standard conditions	10-13 somites		adaxial cell
	IHC	shha^{tbx392/tbx392}	standard conditions	10-13 somites		adaxial cell
	IHC	shha^{tbx392/tbx392} + MO1-myod1	standard conditions	10-13 somites	Not Detected	adaxial cell
	IHC	shha^{tbx392/tbx392} + MO1-myog	standard conditions	10-13 somites	Not Detected	adaxial cell
	IHC	shha^{tbx392/tbx392} + MO3-myf5	standard conditions	10-13 somites		adaxial cell

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT + MO1-myod1	standard conditions	5-9 somites	skeletal muscle tissue development delayed, abnormal
WT + MO1-myog	standard conditions	5-9 somites	skeletal muscle tissue development delayed, abnormal
shha^{tbx392/tbx392}	standard conditions	10-13 somites	skeletal muscle tissue development delayed, abnormal
shha^{tbx392/tbx392} + MO1-myod1	standard conditions	10-13 somites	skeletal muscle tissue development arrested, abnormal
shha^{tbx392/tbx392} + MO1-myog	standard conditions	10-13 somites	skeletal muscle tissue development arrested, abnormal
shha^{tbx392/tbx392} + MO3-myf5	standard conditions	10-13 somites	skeletal muscle tissue development delayed, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.