ZFIN ID: ZDB-FIG-110317-14
Osborn et al., 2011 - Cdkn1c drives muscle differentiation through a positive feedback loop with Myod. Developmental Biology   350(2):464-475 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5

Fig. 4 Cdkn1c expression depends on Hh in tailbud and on MRFs anteriorly MO-injected wild type (B–D) or myf5hu2022 incross embryos (A,B) co-injected with shha mRNA (D,E) analysed for cdkn1c mRNA in 12–15 ss dorsal (A,B,D) or 24 hpf lateral flatmount, dorsal to right (C,E). Anterior is to top. Bars: flatmounts 100 μm, sections 50 μm. (A–C). Adaxial cdkn1c mRNA accumulation initiates independent of MRF activity (arrowheads), but fails to be maintained (bracket). Myod MO reduces cdkn1c expression in older somites (brackets) but tailbud is unaffected (arrowheads). Myf5 and myod knockdown ablates somite cdkn1c mRNA except in small groups of cells lateral to the un-migrated adaxial cells (arrows). Neural, cloacal and transient notochordal expression is unaffected. (D). Over-expression of Shha promotes cdkn1c up-regulation in anterior PSM and somite in contorls and after Myod knockdown, but not in myf5 + myod double morphants (upper brackets). Note that Shha up-regulates cdkn1c in the lateral tailbud of myf5 myod double morphants (lower brackets), but this is not maintained in anterior PSM. (E). Over-expression of Shha promotes ectopic smyhc1 expression at 24 hpf in control or myod morphants, but not in myf5 + myod double morphant embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdkn1ca WT control 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites tail bud ISH
Prim-5 cloacal chamber ISH
Prim-5 neural tube ISH
Prim-5 somite ISH
Prim-5 tail bud ISH
WT + MO1-myod1 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
10-13 somites to 14-19 somites tail bud ISH
WT + MO1-myod1 + MO3-myf5 standard conditions 10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites tail bud ISH
Prim-5 cloacal chamber ISH
Prim-5 neural tube ISH
Prim-5 somite ISH
Prim-5 tail bud ISH
WT + MO3-myf5 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
10-13 somites to 14-19 somites tail bud ISH
myf5hu2022/hu2022 control 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
10-13 somites to 14-19 somites tail bud ISH
myf5hu2022/hu2022 + MO1-myod1 standard conditions 10-13 somites to 14-19 somites Not Detected adaxial cell ISH
10-13 somites to 14-19 somites tail bud ISH
smyhc1 WT control Prim-5 adaxial cell ISH
Prim-5 slow muscle cell ISH
WT + MO1-myod1 standard conditions Prim-5 adaxial cell ISH
Prim-5 slow muscle cell ISH
WT + MO1-myod1 + MO3-myf5 standard conditions Prim-5 Not Detected adaxial cell ISH
Prim-5 Not Detected slow muscle cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
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Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.