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Fig. 5

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ZDB-FIG-110317-15
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Osborn et al., 2011 - Cdkn1c drives muscle differentiation through a positive feedback loop with Myod
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Fig. 5

Cdkn1c rescue of slow myogenesis is Myod-dependent. Slow MyHC (A,B confocal sections) or pan MyHC (C, dorsal flatmount) in embryos lacking Hh signalling. (A). Cdkn1c MO injection reduces residual slow muscle fibres in 24 hpf smob641 mutants. (B). Mosaic over-expression of zebrafish cdkn1c induced by heat-shock of hs70/4:cdkn1cIRESeGFP at 12 ss rescues slow MyHC at 18 ss. Inset shows co-expression of myc-Cdkn1c and eGFP. eGFP mosaicism was ≤ 5%, which corresponds to ~ 36 somitic adaxial cells at 18 ss. Bar: 10 μm; inset 50 μm. (C). Mosaic Xenopus cdkn1 expression rescues adaxial muscle in smob641 mutant or cyA-treated embryos at 10–12 ss. Cdkn1-driven rescue is prevented by myod knockdown. Charts show rescue of slow fibres (left, mean ± sem; Wilcoxon significance test) and fraction of embryos showing rescue (right; n = total embryos). Note that mosaic expression in ~ 10% of adaxial cells would mark ~ 33 somitic slow fibres per embryo at this stage. Bar: 50 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.