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Fig. S3

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ZDB-FIG-110317-21

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Osborn et al., 2011 - Cdkn1c drives muscle differentiation through a positive feedback loop with Myod

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Fig. S3

Cdkn1c knockdown does not affect myod mRNA in presomitic mesoderm

(A). In situ mRNA hybridization for myod alone (left panel) or myod and myog (right panel) in a shha^tbx392/+ incross injected with cdkn1c MOs or vehicle-control. Note the reduced numbers of myog-expressing cells in shha;cdkn1c MO embryo. Dorsal view of flatmounts at 8 som, anterior to top. (B). Western analysis of Myod in 24 hpf embryos injected with cdkn1c MO. A single major band of M_r ~ 44 000 is not reduced relative to β-Tubulin loading control (M_r ~ 55 000). When averaged over three experiments on 16–24 hpf embryos, no significant down-regulation of Myod band was detected after correction for protein loading. Bars: 25 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.