Transgenic Construct

Tg(ZM)

ID

ZDB-TGCONSTRCT-101007-1

Name

Tg(ZM)

Previous Names

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

None

Contains Sequences

UAS (1)

Note

Znomics retroviral construct 5.2kb long. Methods described in Amsterdam and Hopkins 2006 and Jao et al. 2008 The viral construct contains the core viral components, e.g. long terminal repeats (LTRs) and a packaging signal and the gene activation cassette, which includes a polyadenylation signal (pA) from bovine growth hormone, fourteen tandemly repeated UAS14 sequences, and a synthetic element that includes a splice donor (SD).

Plasmid Map

None

Genomic Features

That Utilize Tg(ZM) Construct

Genomic Feature	Affected Genomic Regions
hg8Tg	opa3
ipk1Tg	slc22a21
umc5Tg	ocrl
vu426Tg	cdc14b
vu442Tg	kctd12.1
zf157Tg	tcf7l1b
zm00012575Tg	hes6
zm00057434Tg	brca2
zm00075660Tg	brca2
zm00105646Tg	gnptg
zm00173004Tg	dmrt1
zm00177433Tg	aplnra
zm00260171Tg	ewsr1a
zm00416857Tg	rnpc3
zm00452188Tg	crb2b
zm130198Tg	dcc
zm197263Tg	notch3
zm5961Tg	lta4h

Transgenics

That Utilize Tg(ZM) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
aplnra^{zm00177433Tg/zm00177433Tg}	aplnra	Fig. 1 from Deshwar et al., 2016	Fig. 1 from Deshwar et al., 2016
aplnrb^{hu4145/hu4145}; aplnra^{zm00177433Tg/zm00177433Tg}	aplnra aplnrb	4 figures from Deshwar et al., 2016	4 figures from Deshwar et al., 2016
aplnrb^{hu4145/hu4145}; aplnra^{zm00177433Tg/zm00177433Tg} + MO3-lft1		Fig. 3 from Deshwar et al., 2016	Fig. 3 from Deshwar et al., 2016
brca2^zf1074/+; brca2^{zm00057434Tg/+}	brca2	Fig. 8 from Kroeger et al., 2017	Fig. 8 from Kroeger et al., 2017
brca2^{zm00057434Tg/zm00057434Tg}	brca2	5 figures from Rodriguez-Mari et al., 2011	Fig. 6 from Rodriguez-Mari et al., 2011
brca2^zm00057434Tg	brca2	Fig. 8 from Rodriguez-Mari et al., 2011
cdc14b^{vu426Tg/vu426Tg}	cdc14b	Fig. 1 from Clément et al., 2011
cdc14b^{vu426Tg/vu426Tg} + MO1-cdc14aa		2 figures from Clément et al., 2012
cdc14b^{vu426Tg/vu426Tg} + MO2-cdc14aa		Fig. 2 from Clément et al., 2012
cdc14b^{vu426Tg/vu426Tg}; twu34Tg	cdc14b	Fig. 2 from Clément et al., 2011
cdc14b^{vu426Tg/vu426Tg}; twu34Tg + MO1-cdc14b		Fig. 2 from Clément et al., 2011
crb2b^{zm00452188Tg/zm00452188Tg}	crb2b	3 figures from Ebarasi et al., 2015	Fig. 4 from Ebarasi et al., 2015
dcc^tm272b/+; dcc^zm130198Tg/+ (TL)	dcc	Fig. 2 from Jain et al., 2014
dcc^{zm130198Tg/zm130198Tg} (TL)	dcc	2 figures from Jain et al., 2014	Fig. 1 from Jain et al., 2014
dcc^{zm130198Tg/zm130198Tg}; j1229aGt (TL)	dcc	Fig. 4 from Jain et al., 2014
dcc^{zm130198Tg/zm130198Tg}; ml2Tg	dcc	Fig. 8 from Rosenberg et al., 2014	Fig. 8 from Rosenberg et al., 2014
dmrt1^{zm00173004Tg/+}	dmrt1		Fig. 6 from Webster et al., 2017
dmrt1^{zm00173004Tg/+} + MO1-dnd1			Fig. 6 from Webster et al., 2017
dmrt1^{zm00173004Tg/zm00173004Tg}	dmrt1	2 figures from Webster et al., 2017	2 figures from Webster et al., 2017
dmrt1^{zm00173004Tg/zm00173004Tg} + MO1-dnd1		Fig. 3 from Webster et al., 2017	Fig. 6 from Webster et al., 2017
ewsr1a^{zm00260171Tg/zm00260171Tg}	ewsr1a	3 figures from 2 publications	3 figures from Merkes et al., 2015
gnptg^{zm00105646Tg/+}	gnptg	Fig. 3 from Flanagan-Steet et al., 2016
gnptg^{zm00105646Tg/zm00105646Tg}	gnptg	Fig. 1 from Flanagan-Steet et al., 2016	Fig. 1 from Flanagan-Steet et al., 2016
gnptg^{zm00105646Tg/zm00105646Tg}; y1Tg	gnptg	2 figures from Flanagan-Steet et al., 2016	2 figures from Flanagan-Steet et al., 2016
her1^{hu2124/hu2124}; hes6^{zm00012575Tg/zm00012575Tg}	her1 hes6
hes6^{zm00012575Tg/+} (AB)	hes6		Fig. S1 from Schröter et al., 2010
hes6^{zm00012575Tg/zm00012575Tg}	hes6
hes6^{zm00012575Tg/zm00012575Tg} (AB)	hes6	4 figures from Schröter et al., 2010	4 figures from Schröter et al., 2010
hes6^{zm00012575Tg/zm00012575Tg} + MO1-her1 + MO3-her1 (AB)		Fig. 1 from Schröter et al., 2010	Fig. 1 from Schröter et al., 2010
hes6^{zm00012575Tg/zm00012575Tg} + MO3-hes6 (AB)		Fig. S1 from Schröter et al., 2010	Fig. S1 from Schröter et al., 2010
kctd12.1^{vu442Tg/vu442Tg}	kctd12.1	Fig. 4 from Lee et al., 2014
kctd12.1^{vu442Tg/vu442Tg} (AB)	kctd12.1	Fig. 7 from Taylor et al., 2011	2 figures from Taylor et al., 2011
kctd12.1^{vu442Tg/vu442Tg} + MO2-ulk2 (AB)		Fig. 9 from Taylor et al., 2011	Fig. 9 from Taylor et al., 2011
kctd12.2^fh312/fh312; kctd12.1^{vu442Tg/vu442Tg}	kctd12.1 kctd12.2	Fig. 4 from Lee et al., 2014
kctd12.2^fh312/fh312; kctd12.1^{vu442Tg/vu442Tg} (AB)	kctd12.1 kctd12.2	Fig. 7 from Taylor et al., 2011	Fig. 7 from Taylor et al., 2011
lta4h^{zm5961Tg/zm5961Tg}	lta4h	Fig. S2 from Tobin et al., 2010
notch3^st51/+; notch3^zm197263Tg/+	notch3		Fig. 1 from Zaucker et al., 2013
notch3^zm197263Tg/+	notch3		3 figures from Zaucker et al., 2013
notch3^zm197263Tg/+; y1Tg	notch3		Fig. 2 from Zaucker et al., 2013
notch3^{zm197263Tg/zm197263Tg}	notch3	4 figures from Zaucker et al., 2013	3 figures from Zaucker et al., 2013
notch3^{zm197263Tg/zm197263Tg}; y1Tg	notch3		Fig. 2 from Zaucker et al., 2013
ocrl^{umc5Tg/umc5Tg}	ocrl	20 figures from 5 publications	8 figures from 3 publications
ocrl^{umc5Tg/umc5Tg} (AB)	ocrl	Fig. 5 from Oltrabella et al., 2021
ocrl^{umc5Tg/umc5Tg} + MO1-pheta1 (AB)		Fig. 5 from Oltrabella et al., 2021
ocrl^{umc5Tg/umc5Tg} + MO2-ift88		Fig. 7 from Oltrabella et al., 2015
ocrl^{umc5Tg/umc5Tg}; mw5Tg	ocrl		Fig. 2 from Oltrabella et al., 2015
ocrl^{umc5Tg/umc5Tg}; mw7Tg	ocrl		2 figures from Oltrabella et al., 2015
ocrl^{umc5Tg/umc5Tg}; p152Tg	ocrl	Fig. S3 from Oltrabella et al., 2015	Fig. S3 from Oltrabella et al., 2015
opa3^hg8Tg/hg8Tg	opa3	5 figures from Pei et al., 2010
rnpc3^s846/+; rnpc3^{zm00416857Tg/+}	rnpc3	Fig. S1 from Markmiller et al., 2014
rnpc3^{zm00416857Tg/zm00416857Tg}	rnpc3	Fig. S1 from Markmiller et al., 2014	Fig. S2 from Markmiller et al., 2014
slc22a21^{ipk1Tg/ipk1Tg} (EKW)	slc22a21	2 figures from Pfeiffer et al., 2015
slc22a21^ipk1Tg (EKW)	slc22a21
tcf7l1a^m881/m881; tcf7l1b^{zf157Tg/zf157Tg}	tcf7l1a tcf7l1b	text only from Gribble et al., 2009
tp53^zdf1/+; ewsr1a^{zm00260171Tg/+}	ewsr1a tp53	Fig. 1 from Park et al., 2016
tp53^zdf1/+; ewsr1a^{zm00260171Tg/zm00260171Tg}	ewsr1a tp53	Fig. 1 from Park et al., 2016
tp53^zdf1/zdf1; brca2^{zm00057434Tg/zm00057434Tg}	brca2 tp53	3 figures from Rodriguez-Mari et al., 2011
tp53^zdf1/zdf1; ewsr1a^{zm00260171Tg/+}	ewsr1a tp53	Fig. 1 from Park et al., 2016
tp53^zdf1/zdf1; ewsr1a^{zm00260171Tg/zm00260171Tg}	ewsr1a tp53	Fig. 1 from Park et al., 2016

Sequence Information

Other Construct Pages

None

Citations