Figure 8

FUS Interacts with the mitochondrial anchor protein, Syntaphillin. (A) Representative super-resolution images of rat primary cortical neurons stained for endogenous FUS (green), Syntaphillin (red) and MAP2 (merge). Quantification of the subcellular localisation showed that FUS and SNPH preferentially localised within neurites (N = three dendrites from ten different neurons). Scale bar = 20 μm. Statistical analysis was performed using a paired student’s T-test; error bars are ± SEM. (B) Representative images of PLA in rat primary neurons. PLA (red) was used to assess interactions within soma and neurites which were determined through MAP2 staining (green). Nuclei were counterstained with DAPI. Scale bar = 100 μm (C) Quantification of PLA interactions within soma and neurite for both SNPH. There was an interaction between FUS-SNPH shown in the soma and neurites whilst there appears to be a stronger interaction with FUS-SNPH in the soma when compared to neurites. N = five cells and three neurites per cell were analysed from three different independent replicates. ****p < 0.0001.