ZFIN ID: ZDB-FIG-200423-49
Gong et al., 2020 - The Requirement of Sox2 for the Spinal Cord Motor Neuron Development of Zebrafish. Frontiers in molecular neuroscience   13:34 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Figure 2

Primary motor neuron morphogenesis defects in the Sox2 knockout zebrafish. (A) Confocal imaging analysis of primary motor neurons in control and Sox2 knockout groups at 24, 48, and 72 hpf. The dotted diagonal line marked the boundary of the myosepta. Scale bar = 50 μm. (B) The length of CaP axons in control and Sox2 knockout zebrafish at 48 hpf (n = 21 and 33, respectively) and 72 hpf (n = 13 and 29, respectively). (C) The variability of CaP phenotype across segments in controls and Sox2 mutants at 48 and 72 hpf. Circle means control and triangle means Sox2 mutant. (D) The number of branches per 1-mm CaP axon in control and Sox2 knockout zebrafish at 48 hpf (n = 11 and 9, respectively) and 72 hpf (n = 8 and 12, respectively). Each bar represents the mean ± SD. Values with *, **, and *** above the bars are significantly different (P < 0.05, P < 0.01, and P < 0.001, respectively). (E) The schematic for three different primary motoneurons (CaP, MiP, and RoP) in the whole fish and a specific myotome segment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ml2Tg standard conditions Prim-5 CaP motoneuron IFL
Long-pec CaP motoneuron IFL
Protruding-mouth CaP motoneuron IFL
sox2zf3399/zf3399; ml2Tg standard conditions Prim-5 CaP motoneuron IFL
Long-pec CaP motoneuron IFL
Protruding-mouth CaP motoneuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sox2zf3399/zf3399; ml2Tg standard conditions Prim-5 CaP motoneuron axon decreased length, abnormal
Long-pec CaP motoneuron axon decreased length, abnormal
Long-pec CaP motoneuron increased branchiness, abnormal
Protruding-mouth CaP motoneuron axon decreased length, abnormal
Protruding-mouth CaP motoneuron increased branchiness, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.