ZFIN Figure: Gong et al., 2020, Figure 3

ZFIN ID: ZDB-FIG-200423-50

ZFIN ID: ZDB-FIG-200423-50

Gong et al., 2020 - The Requirement of Sox2 for the Spinal Cord Motor Neuron Development of Zebrafish. Frontiers in molecular neuroscience 13:34 Full text @ Front. Mol. Neurosci.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Gene:	EGFP
Fish:	ml2Tg ml2Tg + MO7-sox2
Knockdown Reagent:	MO7-sox2
Anatomical Term:	primary motor neuron
Stage Range:	Prim-5 to Protruding-mouth

PHENOTYPE:

Fish:	ml2Tg + MO7-sox2
Knockdown Reagent:	MO7-sox2
Observed In:	primary motor neuron
Stage Range:	Prim-5 to Protruding-mouth

Figure 3

Primary motor neuron developmental defects in the Sox2 morphants. (A) Confocal imaging analysis of primary motor neurons in wild type and Sox2 morphants at 24, 48, and 72 hpf. Scale bar = 50 μm. (B) Quantification of zebrafish embryos with abnormal PMNs (n = 176 and 238, respectively). (C) The length of CaP axons in control and Sox2 morphants at 48 hpf (n = 21 and 32, respectively) and 72 hpf (n = 17 and 19, respectively). (D) The number of branches per 1-mm CaP axon in control and Sox2 morphants at 48 (n = 8 and 7, respectively) and 72 (n = 5 and 6, respectively) hpf. Each bar represents the mean ± SD. Values with ** and *** above the bars are significantly different (P < 0.01 and P < 0.001, respectively).

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
EGFP	ml2Tg	control	Prim-5	primary motor neuron	IFL
			Long-pec	primary motor neuron	IFL
			Protruding-mouth	primary motor neuron	IFL
	ml2Tg + MO7-sox2	control	Prim-5	primary motor neuron	IFL
			Long-pec	primary motor neuron	IFL
			Protruding-mouth	primary motor neuron	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
ml2Tg + MO7-sox2	control	Prim-5	primary motor neuron decreased length, abnormal
		Long-pec	primary motor neuron decreased length, abnormal
		Long-pec	primary motor neuron increased branchiness, abnormal
		Long-pec	primary motor neuron morphology, abnormal
		Protruding-mouth	primary motor neuron decreased length, abnormal
		Protruding-mouth	primary motor neuron increased branchiness, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.