ZFIN ID: ZDB-FIG-200423-50
Gong et al., 2020 - The Requirement of Sox2 for the Spinal Cord Motor Neuron Development of Zebrafish. Frontiers in molecular neuroscience   13:34 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Figure 3

Primary motor neuron developmental defects in the Sox2 morphants. (A) Confocal imaging analysis of primary motor neurons in wild type and Sox2 morphants at 24, 48, and 72 hpf. Scale bar = 50 μm. (B) Quantification of zebrafish embryos with abnormal PMNs (n = 176 and 238, respectively). (C) The length of CaP axons in control and Sox2 morphants at 48 hpf (n = 21 and 32, respectively) and 72 hpf (n = 17 and 19, respectively). (D) The number of branches per 1-mm CaP axon in control and Sox2 morphants at 48 (n = 8 and 7, respectively) and 72 (n = 5 and 6, respectively) hpf. Each bar represents the mean ± SD. Values with ** and *** above the bars are significantly different (P < 0.01 and P < 0.001, respectively).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ml2Tg control Prim-5 primary motor neuron IFL
Long-pec primary motor neuron IFL
Protruding-mouth primary motor neuron IFL
ml2Tg + MO7-sox2 control Prim-5 primary motor neuron IFL
Long-pec primary motor neuron IFL
Protruding-mouth primary motor neuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ml2Tg + MO7-sox2 control Prim-5 primary motor neuron decreased length, abnormal
Long-pec primary motor neuron decreased length, abnormal
Long-pec primary motor neuron increased branchiness, abnormal
Long-pec primary motor neuron morphology, abnormal
Protruding-mouth primary motor neuron decreased length, abnormal
Protruding-mouth primary motor neuron increased branchiness, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.