ZFIN ID: ZDB-FIG-200423-48
Gong et al., 2020 - The Requirement of Sox2 for the Spinal Cord Motor Neuron Development of Zebrafish. Frontiers in molecular neuroscience   13:34 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-25

Figure 1

Sex-determining region Y box 2 (Sox2) expression analyses in the spinal cord and motor neurons. (A) At 20 hpf, the in situ hybridization signal of Sox2 is localized in the spinal cord. (A′) The magnified figure of the region squared in dashed line. (A″) The trunk transverse section of the embryos. (B) Sox2 is expressed in the spinal cord and the deposited neuromasts. (B′) The magnified figure of the region squared in dashed line. Scale bar = 200 μm. (C) The procedure of the motor neurons and endothelial cells sorting and RT-PCR. (D) The results of the RT-PCR on Mnx1-GFP sorted cells. Sox2 is expressed in the selected neuron cells from the Tg(Mnx1:EGFP) line. (E) The result of the RT-PCR on Kdrl-EGFP sorted cells. No signals of Sox2 and mnx1 are detected in the GFP-positive cells sorted from the Tg(kdrl:EGFP) line.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mnx1 ml2Tg standard conditions Prim-5 motor neuron RTPCR
s843Tg standard conditions Prim-5 Not Detected endothelial cell RTPCR
sox2 WT standard conditions 20-25 somites brain ISH
20-25 somites spinal cord neural tube ISH
Prim-25 neuromast ISH
Prim-25 posterior lateral line primordium ISH
Prim-25 spinal cord ISH
ml2Tg standard conditions Prim-5 motor neuron RTPCR
s843Tg standard conditions Prim-5 Not Detected endothelial cell RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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