Sex-determining region Y box 2 (Sox2) expression analyses in the spinal cord and motor neurons. (A) At 20 hpf, the in situ hybridization signal of Sox2 is localized in the spinal cord. (A′) The magnified figure of the region squared in dashed line. (A″) The trunk transverse section of the embryos. (B) Sox2 is expressed in the spinal cord and the deposited neuromasts. (B′) The magnified figure of the region squared in dashed line. Scale bar = 200 μm. (C) The procedure of the motor neurons and endothelial cells sorting and RT-PCR. (D) The results of the RT-PCR on Mnx1-GFP sorted cells. Sox2 is expressed in the selected neuron cells from the Tg(Mnx1:EGFP) line. (E) The result of the RT-PCR on Kdrl-EGFP sorted cells. No signals of Sox2 and mnx1 are detected in the GFP-positive cells sorted from the Tg(kdrl:EGFP) line.