PUBLICATION

The Requirement of Sox2 for the Spinal Cord Motor Neuron Development of Zebrafish

Authors
Gong, J., Hu, S., Huang, Z., Hu, Y., Wang, X., Zhao, J., Qian, P., Wang, C., Sheng, J., Lu, X., Wei, G., Liu, D.
ID
ZDB-PUB-200422-124
Date
2020
Source
Frontiers in molecular neuroscience   13: 34 (Journal)
Registered Authors
Gong, Jie, Liu, Dong
Keywords
Sox2, axons, development, differentiation, motor neuron, zebrafish
MeSH Terms
none
PubMed
32292330 Full text @ Front. Mol. Neurosci.
Abstract
Sex-determining region Y box 2 (Sox2), expressed in neural tissues, plays an important role as a transcription factor not only in the pluripotency and proliferation of neuronal cells but also in the opposite function of cell differentiation. Nevertheless, how Sox2 is linked to motor neuron development remains unknown. Here, we showed that Sox2 was localized in the motor neurons of spinal cord by in situ hybridization and cell separation, which acted as a positive regulator of motor neuron development. The deficiency of Sox2 in zebrafish larvae resulted in abnormal PMN development, including truncated but excessively branched CaP axons, loss of MiP, and increase of undifferentiated neuron cells. Importantly, transcriptome analysis showed that Sox2-depleted embryos caused many neurogenesis, axonogenesis, axon guidance, and differentiation-related gene expression changes, which further support the vital function of Sox2 in motor neuron development. Taken together, these data indicate that Sox2 plays a crucial role in the motor neuron development by regulating neuron differentiation and morphology of neuron axons.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes