FIGURE

Fig. 7

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ZDB-FIG-180427-28
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Hui et al., 2017 - Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs
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Fig. 7

Impaired Regeneration in Foxp3a-Deficient Zebrafish

(A) Foxp3a domain structure and predicted truncated product of the Foxp3avcc6 allele. Numbers indicate amino acid positions.

(B) Sections of Foxp3a-deficient (foxp3a/) and WT (foxp3a+/+) clutch-mate foxp3a:RFP fish. Asterisks indicate injury epicenter.

(C–E) Proliferation of newborn neurons (C), cardiomyocytes (D), and Müller glia (E). Insets show single-channel confocal slices (C and E) or epifluorescent images (D) of the demarcated regions. Arrows mark co-labeled cells. Dotted lines indicate the wound border.

(F–H) Quantification of (C), (D) and (E), respectively (mean ± SEM, n = 5–7).

(I–K) qRT-PCR analysis of regeneration factor genes (mean ± SEM, n = 6).

(L–N) Semi-qRT-PCR analysis of regeneration factor genes in purified foxp3a:RFP+ cells. +/+, foxp3a+/+; −/−, foxp3a/.

Confocal projections of z stacks are shown, except for the epifluorescent images in (D). p < 0.05, ∗∗p < 0.01, Mann-Whitney U test. Scale bars, 50 μm. See also Figure S7.

Expression Data
Genes:
Antibodies:
Fish:
Conditions:
Anatomical Terms:
Stage: Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Conditions:
Observed In:
Stage: Adult

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Cell, 43, Hui, S.P., Sheng, D.Z., Sugimoto, K., Gonzalez-Rajal, A., Nakagawa, S., Hesselson, D., Kikuchi, K., Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs, 659-672.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell