ZFIN ID: ZDB-FIG-180201-2
Gong et al., 2017 - Insm1a Regulates Motor Neuron Development in Zebrafish. Frontiers in molecular neuroscience   10:274 Full text @ Front. Mol. Neurosci.
Knockdown Reagent:
Observed In:
Stage: Days 14-20

Fig. S2

Generation of zebrafish insm1a mutant using CRISPR/Cas9 system. A. Schematic diagram showing the targeting site of the sgRNA on the first exon of insm1a gene. Starting codon (ATG) site is indicated by arrow. B. Mutation pattern of insm1a-gRNA/cas9-injecting embryos. Numbers in the brackets show the numbers of nucleotides were deleted (−). C. Schematic diagram showing the predicted proteins encoded by the three mutated alleles. The mutants are reading frameshift mutations that result in truncated proteins. The gray rectangles indicate the wrong coded amino acid sequences. D. The imaging analysis of insm1a mutant and wide type zebrafish at 48 hpf in bright field. E. Death rate of zebrafish embryos in control group and insm1a mutant groups at 15dpf.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ml2Tg + CRISPR1-insm1a standard conditions Days 14-20 whole organism decreased life span, abnormal
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.