ZFIN ID: ZDB-FIG-180131-29
Gong et al., 2017 - Insm1a Regulates Motor Neuron Development in Zebrafish. Frontiers in molecular neuroscience   10:274 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 3

Insm1a deficiency suppressed neuronal cells differentiation. (A) Confocal imaging analysis of primary motor neuron in control group, insm1a mutant group and morphant group at 30 and 48 hpf Tg(mnx1:GFP)ml2. Phenotypes of neuronal cells in the spinal cord in control group, morphant group, and insm1a mutant groups at 30 hpf and 48 hpf. Asterisks indicate undifferentiated neuronal cells. Scale bar = 50 μm. (B) Quantification of the undifferentiated neuronal cell in the insm1a different treatment zebrafish. Asterisks above the bars indicate significant differences (*P < 0.05, **P < 0.01). (C) Time-lapse imaging analysis of the primary motor neuron in control group and insm1a mutant groups. Asterisks represent undifferentiated neuronal cells. Scale bar = 50 μm

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ml2Tg + CRISPR1-insm1a standard conditions Prim-15 neuron differentiation decreased process quality, abnormal
Prim-15 neuron undifferentiated, abnormal
Long-pec neuron differentiation decreased process quality, abnormal
Long-pec neuron undifferentiated, abnormal
ml2Tg + MO5-insm1a standard conditions Prim-15 neuron differentiation decreased process quality, abnormal
Prim-15 neuron undifferentiated, abnormal
Long-pec neuron differentiation decreased process quality, abnormal
Long-pec neuron undifferentiated, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.