ZFIN ID: ZDB-FIG-180201-4
Gong et al., 2017 - Insm1a Regulates Motor Neuron Development in Zebrafish. Frontiers in molecular neuroscience   10:274 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. S4

Primary motor neuron developmental defects in the insm1a morphants. A. Confocal imaging analysis of primary motor neuron in control group and insm1a morpholino injected groups at 30 hpf and 48 hpf Tg(mnx1:GFP)ml2. Caps in dash line are showed in diagrams. Scale bar = 50 μ m. B. Quantification of distance between adjacent motor neurons (μm) in control group and insm1a morpholino injection groups at 30 hpf (n=23 and 41 respectively) and 48 hpf (n=26 and 51 respectively). C and D. The length and branching number of Cap axons in control group and insm1a morphant groups at 30 hpf and 48 hpf. Asterisks above the bars are significantly different (P<0.05). Values with ** above the bars are significantly different (P<0.05). E. Abnormal Caps in insm1a knockdown zebrafish embryos were restored by co-injection of insm1a mRNA with the morpholino. F. Quantification of zebrafish embryos with abnormal Caps.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ml2Tg + MO5-insm1a standard conditions Prim-15 CaP motoneuron decreased amount, abnormal
Prim-15 CaP motoneuron distance CaP motoneuron, abnormal
Prim-15 MiP motor neuron decreased amount, abnormal
Long-pec CaP motoneuron axon decreased length, abnormal
Long-pec CaP motoneuron axon increased branchiness, abnormal
Long-pec CaP motoneuron decreased amount, abnormal
Long-pec CaP motoneuron distance CaP motoneuron, abnormal
Long-pec MiP motor neuron decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.