ZFIN ID: ZDB-FIG-180131-28
Gong et al., 2017 - Insm1a Regulates Motor Neuron Development in Zebrafish. Frontiers in molecular neuroscience   10:274 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 2

Primary motor neuron morphogenesis defects in the insm1a mutant zebrafish embryos. (A) Confocal imaging analysis of primary motor neuron in control group and insm1a mutant groups at 30 hpf and 48 hpf Tg(mnx1:GFP)ml2. Caps in dash line are showed in diagrams. Scale bar = 50 μm. (B) Quantification of zebrafish embryos with abnormal Caps. The zebrafish embryos are classified into three categories according to its loss degree: severe group with over 80% loss of Cap primary motor neuron, moderate group with <80% loss, and normal group with <20% loss. (C) Quantification of distance between adjacent motor neurons (μm) in control group and insm1a mutant groups at 30 hpf (n = 33 and 41 respectively) and 48 hpf (n = 27 and 76 respectively). (D,E) The length and branching number of Cap axons in control group and insm1a mutant groups at 30 and 48 hpf. Asterisks above the bars indicate significant differences (**P < 0.01). (F) Quantification of zebrafish embryos with abnormal Caps at 30 and 48 hpf.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ml2Tg + CRISPR1-insm1a standard conditions Prim-15 CaP motoneuron axon decreased length, abnormal
Prim-15 CaP motoneuron decreased amount, abnormal
Prim-15 CaP motoneuron distance CaP motoneuron, abnormal
Prim-15 MiP motor neuron decreased amount, abnormal
Long-pec CaP motoneuron axon decreased length, abnormal
Long-pec CaP motoneuron axon increased branchiness, abnormal
Long-pec CaP motoneuron decreased amount, abnormal
Long-pec CaP motoneuron distance CaP motoneuron, abnormal
Long-pec MiP motor neuron decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.