FIGURE

Fig. S2

ID

ZDB-FIG-171228-51

Publication

Rességuier et al., 2017 - Specific and Efficient Uptake of Surfactant-Free Poly(Lactic Acid) Nanovaccine Vehicles by Mucosal Dendritic Cells in Adult Zebrafish after Bath Immersion

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Fig. S2

Artefactual nanoparticle displacement at cryosection surface. (A, B): Images of 40µm-thick gut cryosections from adult zebrafish exposed for 24h to 0.05% red fluorescent nanoparticles. Pictures are maximal intensity projection of 5 confocal pictures taken every two micrometers. Whereas acquisitions in the core of the cryosection reveal low nanoparticle uptake in the lamina propria (A), a larger number of nanoparticles are detected in the same region at the surface the cryosection (B), suggesting that PLA nanoparticles may be displaced during immersion steps of the staining protocol. (C): Epifluorescence image from similar cryosections, cut across a nanoparticle-rich region of the gut lumen. The smear of nanoparticles originating from the lumen suggests that the cryosecting blade may displace nanoparticles at the surface of sections. Lu (Lumen), Lp (Lamina propria), Ep (Epithelium), I (Intestines), P (Pancreas), L (Liver). Scale bar: 10 µm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol