FIGURE

Fig. 2

ID

ZDB-FIG-171228-47

Publication

Rességuier et al., 2017 - Specific and Efficient Uptake of Surfactant-Free Poly(Lactic Acid) Nanovaccine Vehicles by Mucosal Dendritic Cells in Adult Zebrafish after Bath Immersion

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Fig. 2

Poly(lactic acid) nanoparticles (PLA-NPs) are accumulated in mucosal antigen-presenting cells. (A–D) Representative confocal images of gills (A–C) and skin (D) of wild-type adults immersed for 24 h in 0.01% fluorescent PLA-NPs (red). Images were acquired from 40-μm-thick whole-body cryosections, stained for neutrophils (Mpx-positive, cyan) (A), macrophages (Mpeg1-positive, cyan) (B), IgZ⁺ B-cells (green) (C), and dendritic cells (DCs) (PNA, green) (A,B,D), in addition to nuclei (DAPI, blue). Maximal intensity projections from 3 (A–C) or 5 (D) optic sections acquired every micrometer. Nanoparticle (NP) accumulations are observed in gill DCs [(A,B), arrows], macrophages [(B), star], and IgZ⁺ cells [(C), arrow], while no NP are detected in neutrophils [(A), stars]. NPs are also taken up by a network of skin DCs [(D), arrows]. pL, primary lamellae; sL, secondary lamellae; Bc, branchial cavity; B, blood; C, cartilage; Ext, external environment; E, epidermis. Scale bar: 10 μm.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol