ZFIN ID: ZDB-FIG-170502-5
Zhang et al., 2017 - Critical Role for GAB2 in Neuroblastoma Pathogenesis through the Promotion of SHP2/MYCN Cooperation. Cell Reports   18:2932-2942 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Condition:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 6

Targeting MYCN with CBL0137 Is Effective against Neuroblastoma in MYCN;GFP Transgenic Fish

(A–D) EGFP-expressing tumors (arrowhead) in the MYCN;GFP compound transgenic fish, treated with vehicle (A and B) or CBL0137 (C and D) at days 1 or 15 of treatment. Scale bar, 1 mm.

(E) Differences in tumor volume in animals treated with vehicle or CBL0137 on day 15 versus day 1, as measured by the size of EGFP-positive tumor masses using ImageJ software. Mean values (horizontal bars) were compared by Welch t test (two-tailed).

(F) Histopathologic and immunohistochemical analyses of MYCN;GFP tumors treated with vehicle or CBL0137. Left: H&E-stained sagittal sections. Right: immunohistochemical staining with PCNA antibody. Scale bar, 50 μm.

(G) Immunoblot of lysates from MYCN;GFP tumors treated with vehicle or CBL0137. Total levels of Erk1/2 serve as loading controls.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zdf16Tg (AB) control Adult interrenal gland neuroblastoma IFL
Adult interrenal gland neuroblastoma WB
zdf16Tg (AB) chemical treatment by injection: CBL0137 Adult interrenal gland neuroblastoma IFL
Adult interrenal gland neuroblastoma WB
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ssrp1 WB zdf16Tg (AB) control Adult interrenal gland neuroblastoma
WB zdf16Tg (AB) chemical treatment by injection: CBL0137 Adult interrenal gland neuroblastoma
Ab1-supt16h WB zdf16Tg (AB) control Adult interrenal gland neuroblastoma
WB zdf16Tg (AB) chemical treatment by injection: CBL0137 Adult interrenal gland neuroblastoma
Ab5-pcna IHC zdf16Tg (AB) control Adult interrenal gland neuroblastoma
IHC zdf16Tg (AB) chemical treatment by injection: CBL0137 Adult interrenal gland neuroblastoma
Ab6-mapk WB zdf16Tg (AB) control Adult interrenal gland neuroblastoma
WB zdf16Tg (AB) chemical treatment by injection: CBL0137 Adult interrenal gland neuroblastoma
Phenotype Details
Fish Conditions Stage Phenotype
zdf16Tg (AB) control Adult interrenal gland neuroblastoma increased amount, abnormal
zdf16Tg (AB) chemical treatment by injection: CBL0137 Adult interrenal gland neuroblastoma Ab1-ssrp1 labeling decreased amount, abnormal
Adult interrenal gland neuroblastoma Ab1-supt16h labeling decreased amount, abnormal
Adult interrenal gland neuroblastoma ab5-pcna labeling increased amount, abnormal
Adult interrenal gland neuroblastoma EGFP expression decreased amount, abnormal
Adult interrenal gland neuroblastoma increased amount, ameliorated
Adult interrenal gland neuroblastoma volume, ameliorated
Acknowledgments:
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