|ZFIN ID: ZDB-FIG-170502-4|
Overexpression of Ptpn11mut Inhibits the Developmentally Timed Apoptotic Response Triggered by MYCN Overexpression in the IRG
(A) Sagittal sections through the IRG of MYCN (top) and MYCN;ptpn11mut (bottom) transgenic fish at 5.5 wpf (dorsal up, anterior left). GFP, green; activated caspase-3, red; DAPI, blue. Arrowheads point to the activated caspase-3+ apoptotic cells. Dotted lines indicate the head kidney (HK) boundary. Scale bars, 10 μm.
(B) Percentage of activated caspase-3+ apoptotic cells to the total number of GFP+ sympathetic neuroblasts in the IRG of MYCN and MYCN;ptpn11mut fish at 5.5 wpf. Mean values (horizontal bars) were compared by the Welch t test (two-tailed).
|EGFP||zdf16Tg (AB)||standard conditions||Days 30-44||IHC|
|zdf16Tg; zf738Tg (AB)||standard conditions||Days 30-44||IHC|
|Ab1-casp3||IHC||zdf16Tg (AB)||standard conditions||Days 30-44|
|IHC||zdf16Tg; zf738Tg (AB)||standard conditions||Days 30-44|
|zdf16Tg; zf738Tg (AB)||standard conditions||Days 30-44||interrenal gland neuron apoptotic process decreased rate, abnormal|
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