ZFIN ID: ZDB-FIG-170502-4
Zhang et al., 2017 - Critical Role for GAB2 in Neuroblastoma Pathogenesis through the Promotion of SHP2/MYCN Cooperation. Cell Reports   18:2932-2942 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage: Days 30-44
PHENOTYPE:
Fish:
Observed In:
Stage: Days 30-44

Fig. 5

Overexpression of Ptpn11mut Inhibits the Developmentally Timed Apoptotic Response Triggered by MYCN Overexpression in the IRG

(A) Sagittal sections through the IRG of MYCN (top) and MYCN;ptpn11mut (bottom) transgenic fish at 5.5 wpf (dorsal up, anterior left). GFP, green; activated caspase-3, red; DAPI, blue. Arrowheads point to the activated caspase-3+ apoptotic cells. Dotted lines indicate the head kidney (HK) boundary. Scale bars, 10 μm.

(B) Percentage of activated caspase-3+ apoptotic cells to the total number of GFP+ sympathetic neuroblasts in the IRG of MYCN and MYCN;ptpn11mut fish at 5.5 wpf. Mean values (horizontal bars) were compared by the Welch t test (two-tailed).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zdf16Tg (AB) standard conditions Days 30-44 interrenal gland neuroblast IHC
zdf16Tg; zf738Tg (AB) standard conditions Days 30-44 interrenal gland neuroblast IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-casp3 IHC zdf16Tg (AB) standard conditions Days 30-44 interrenal gland neuroblast
IHC zdf16Tg; zf738Tg (AB) standard conditions Days 30-44 interrenal gland neuroblast
Phenotype Details
Fish Conditions Stage Phenotype
zdf16Tg; zf738Tg (AB) standard conditions Days 30-44 interrenal gland neuron apoptotic process decreased rate, abnormal
Acknowledgments:
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