| ZFIN ID: ZDB-FIG-170502-4 |
| Gene: | |
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| Antibody: | |
| Fish: | |
| Anatomical Term: | |
| Stage: | Days 30-44 |
| Fish: | |
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| Observed In: | |
| Stage: | Days 30-44 |
Fig. 5 Overexpression of Ptpn11mut Inhibits the Developmentally Timed Apoptotic Response Triggered by MYCN Overexpression in the IRG (A) Sagittal sections through the IRG of MYCN (top) and MYCN;ptpn11mut (bottom) transgenic fish at 5.5 wpf (dorsal up, anterior left). GFP, green; activated caspase-3, red; DAPI, blue. Arrowheads point to the activated caspase-3+ apoptotic cells. Dotted lines indicate the head kidney (HK) boundary. Scale bars, 10 μm. (B) Percentage of activated caspase-3+ apoptotic cells to the total number of GFP+ sympathetic neuroblasts in the IRG of MYCN and MYCN;ptpn11mut fish at 5.5 wpf. Mean values (horizontal bars) were compared by the Welch t test (two-tailed). |
| Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
|---|---|---|---|---|---|---|
| EGFP | zdf16Tg (AB) | standard conditions | Days 30-44 | interrenal gland neuroblast | IHC | |
| zdf16Tg; zf738Tg (AB) | standard conditions | Days 30-44 | interrenal gland neuroblast | IHC |
| Antibody | Assay | Fish | Conditions | Stage | Anatomy |
|---|---|---|---|---|---|
| Ab1-casp3 | IHC | zdf16Tg (AB) | standard conditions | Days 30-44 | interrenal gland neuroblast |
| IHC | zdf16Tg; zf738Tg (AB) | standard conditions | Days 30-44 | interrenal gland neuroblast |
| Fish | Conditions | Stage | Phenotype |
|---|---|---|---|
| zdf16Tg; zf738Tg (AB) | standard conditions | Days 30-44 | interrenal gland neuron apoptotic process decreased rate, abnormal |
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