ZFIN ID: ZDB-FIG-170502-3
Zhang et al., 2017 - Critical Role for GAB2 in Neuroblastoma Pathogenesis through the Promotion of SHP2/MYCN Cooperation. Cell Reports   18:2932-2942 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Days 30-44
PHENOTYPE:
Fish:
Observed In:
Stage: Days 30-44

Fig. 4

Mutant ptpn11 Enhances the Proliferation of MYCN-Induced Hyperplastic Neuroblasts in the IRG

(A) Sagittal sections through the IRGs of control Dβh, ptpn11mut, MYCN, and MYCN;ptpn11mut transgenic fish at 5 wpf (dorsal up, anterior left). GFP, green; Hu, red; DAPI, blue; merge, combined green, red, and blue. Representative sections through the IRG of control Dβh or ptpn11mut fish contain 1-5 GFP+/Hu+ sympathetic neuroblasts (arrowheads). Hu+ cell numbers increase in MYCN and MYCN;ptpn11mut fish (brackets). Dotted lines indicate the head kidney (HK) boundary. Scale bar, 20 μm.

(B) Numbers of GFP+/Hu+ sympathetic neuroblasts in the IRG regions of control Dβh, ptpn11mut, MYCN, and MYCN;ptpn11mut transgenic fish at 5 wpf.

(C) Percentage of EdU+/Hu+ sympathetic neuroblasts in control Dβh, ptpn11mut, MYCN, and MYCN;ptpn11mut transgenic fish at 5 weeks. Mean values (horizontal bars) were compared by Welch t test (two-tailed).

See also Figures S3 and S4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zdf16Tg (AB) standard conditions Days 30-44 interrenal gland neuroblast IHC
Days 30-44 interrenal gland sympathetic neuron IHC
zdf16Tg; zf738Tg (AB) standard conditions Days 30-44 interrenal gland neuroblast IHC
Days 30-44 interrenal gland sympathetic neuron IHC
zdf22Tg (AB) standard conditions Days 30-44 interrenal gland neuroblast IHC
Days 30-44 interrenal gland sympathetic neuron IHC
zf738Tg (AB) standard conditions Days 30-44 interrenal gland neuroblast IHC
Days 30-44 interrenal gland sympathetic neuron IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC zdf16Tg (AB) standard conditions Days 30-44 interrenal gland neuroblast
IHC Days 30-44 interrenal gland sympathetic neuron
IHC zdf16Tg; zf738Tg (AB) standard conditions Days 30-44 interrenal gland neuroblast
IHC Days 30-44 interrenal gland sympathetic neuron
IHC zdf22Tg (AB) standard conditions Days 30-44 interrenal gland neuroblast
IHC Days 30-44 interrenal gland sympathetic neuron
IHC zf738Tg (AB) standard conditions Days 30-44 interrenal gland neuroblast
IHC Days 30-44 interrenal gland sympathetic neuron
Phenotype Details
Fish Conditions Stage Phenotype
zdf16Tg (AB) standard conditions Days 30-44 interrenal gland neural precursor cell proliferation increased rate, abnormal
Days 30-44 interrenal gland neuroblast increased amount, abnormal
zdf16Tg; zf738Tg (AB) standard conditions Days 30-44 interrenal gland neural precursor cell proliferation increased rate, exacerbated
Days 30-44 interrenal gland neuroblast increased amount, exacerbated
Acknowledgments:
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