ZFIN ID: ZDB-FIG-170502-1
Zhang et al., 2017 - Critical Role for GAB2 in Neuroblastoma Pathogenesis through the Promotion of SHP2/MYCN Cooperation. Cell Reports   18:2932-2942 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Term:
Stage Range: Days 30-44 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 30-44 to Adult

Fig. 1

Mutant ptpn11 Synergizes with MYCN in Neuroblastomagenesis

(A–D) Fluorescence micrographs of control Dβh (A), ptpn11mut (B), MYCN (C), and compound MYCN;ptpn11mut (D) transgenic fish. Note the EGFP-expressing tumor in the interrenal gland (IRG; arrowhead in D) at 5 weeks postfertilization (wpf). Scale bar, 1 mm.

(E) Kaplan-Meier analysis showing cumulative frequency of neuroblastoma induction over 6 months in the indicated stable transgenic zebrafish lines (p < 0.0001 for MYCN-only versus MYCN;ptpn11mut by log-rank test).

(F) Phospho-Erk1/2 immunoblots of lysates from individual MYCN-only (F1–F4) or MYCN;ptpn11mut tumors (F5–F8). Total Erk1/2 levels serve as a loading control.

(G) Kaplan-Meier analysis of cumulative frequency of neuroblastoma induction over 11 weeks in MYCN transgenic fish mosaically overexpressing mCherry alone (MYCN;mosaic mCherry), coexpressing mCherry with ptpn11wt (MYCN;mosaic ptpn11wt), or coexpressing mCherry with ptpn11mut (MYCN;mosaic ptpn11mut). MYCN;mosaic ptpn11mut versus MYCN;mosaic mCherry transgenic fish is significant by log-rank test (p = 0.03).

See also Figure S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zdf16Tg; zf738Tg (AB) standard conditions Days 30-44 interrenal gland neuroblastoma IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ptpn11 WB zdf16Tg (AB) standard conditions Adult interrenal gland neuroblastoma
WB zdf16Tg; zf738Tg (AB) standard conditions Adult interrenal gland neuroblastoma
Ab6-mapk WB zdf16Tg (AB) standard conditions Adult interrenal gland neuroblastoma
WB zdf16Tg; zf738Tg (AB) standard conditions Adult interrenal gland neuroblastoma
Phenotype Details
Fish Conditions Stage Phenotype
zdf16Tg (AB) standard conditions Days 30-44 - Adult interrenal gland neuroblastoma increased amount, abnormal
Adult interrenal gland neuroblastoma Ab1-ptpn11 labeling increased amount, abnormal
zdf16Tg; zf738Tg (AB) standard conditions Days 30-44 - Adult interrenal gland neuroblastoma increased amount, exacerbated
Adult interrenal gland neuroblastoma Ab1-ptpn11 labeling increased amount, abnormal
Adult interrenal gland neuroblastoma ab6-mapk labeling increased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.