ZFIN ID: ZDB-FIG-170502-6
Zhang et al., 2017 - Critical Role for GAB2 in Neuroblastoma Pathogenesis through the Promotion of SHP2/MYCN Cooperation. Cell Reports   18:2932-2942 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Conditions:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Adult

Fig. 7

Erk Inhibition Cooperates with CBL0137 to Suppress MYCN;Gab2wt Neuroblastoma

(A–F) EGFP-expressing tumors (arrowhead) in the MYCN;Gab2wt transgenic fish treated with vehicle (A and B), CBL0137 alone (C and D), or CBL0137 plus trametinib (E and F) at day 1 or day 15 of treatment. Scale bar, 1 mm.

(G) Differences in tumor volume in animals treated with vehicle, CBL0137, or CBL0137 plus trametinib on day 15 versus day 1, as measured by the size of EGFP-positive tumor masses using ImageJ software. Mean values (horizontal bars) were compared by Welch t test (two-tailed).

(H) Histopathologic and immunohistochemical analyses of MYCN;Gab2wt tumors treated with vehicle or combined CBL0137 and trametinib. Left: H&E-stained sagittal sections. Right: immunohistochemical staining with PCNA antibody. Scale bar, 50 μm.

(I) Immunoblot of lysates from MYCN;Gab2wt tumors treated with vehicle or CBL0137 and trametinib. Total levels of Erk1/2 serve as loading controls.

See also Figures S5 and S6.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zdf16Tg; zf739Tg (AB) control Adult interrenal gland neuroblastoma WB
Adult interrenal gland neuroblastoma IFL
zdf16Tg; zf739Tg (AB) chemical treatment by injection: CBL0137 Adult interrenal gland neuroblastoma IFL
zdf16Tg; zf739Tg (AB) chemical treatment by injection: trametinib, chemical treatment by injection: CBL0137 Adult interrenal gland neuroblastoma WB
Adult interrenal gland neuroblastoma IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ssrp1 WB zdf16Tg; zf739Tg (AB) control Adult interrenal gland neuroblastoma
WB zdf16Tg; zf739Tg (AB) chemical treatment by injection: trametinib, chemical treatment by injection: CBL0137 Adult interrenal gland neuroblastoma
Ab1-supt16h WB zdf16Tg; zf739Tg (AB) control Adult interrenal gland neuroblastoma
WB zdf16Tg; zf739Tg (AB) chemical treatment by injection: trametinib, chemical treatment by injection: CBL0137 Adult interrenal gland neuroblastoma
Ab5-pcna IHC zdf16Tg; zf739Tg (AB) control Adult interrenal gland neuroblastoma
IHC zdf16Tg; zf739Tg (AB) chemical treatment by injection: trametinib, chemical treatment by injection: CBL0137 Adult interrenal gland neuroblastoma
Ab6-mapk WB zdf16Tg; zf739Tg (AB) control Adult interrenal gland neuroblastoma
WB zdf16Tg; zf739Tg (AB) chemical treatment by injection: trametinib, chemical treatment by injection: CBL0137 Adult interrenal gland neuroblastoma
Phenotype Details
Fish Conditions Stage Phenotype
zdf16Tg; zf739Tg (AB) control Adult interrenal gland neuroblast increased amount, abnormal
zdf16Tg; zf739Tg (AB) chemical treatment by injection: CBL0137 Adult interrenal gland neuroblast increased amount, abnormal
zdf16Tg; zf739Tg (AB) chemical treatment by injection: trametinib, chemical treatment by injection: CBL0137 Adult interrenal gland neuroblastoma Ab1-ssrp1 labeling decreased amount, abnormal
Adult interrenal gland neuroblastoma Ab1-supt16h labeling decreased amount, abnormal
Adult interrenal gland neuroblastoma ab5-pcna labeling decreased amount, abnormal
Adult interrenal gland neuroblastoma ab6-mapk labeling decreased amount, abnormal
Adult interrenal gland neuroblastoma EGFP expression decreased amount, abnormal
Adult interrenal gland neuroblastoma increased amount, ameliorated
Adult interrenal gland neuroblastoma volume, ameliorated
Acknowledgments:
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