ZFIN ID: ZDB-FIG-170502-2
Zhang et al., 2017 - Critical Role for GAB2 in Neuroblastoma Pathogenesis through the Promotion of SHP2/MYCN Cooperation. Cell Reports   18:2932-2942 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Term:
Stage Range: Days 21-29 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 21-29 to Adult

Fig. 3

GAB2 and MYCN Synergize in Neuroblastomagenesis

(A and B) Fluorescence micrographs of MYCN (A) and compound MYCN;Gab2wt (B) transgenic fish. Note EGFP-expressing tumor in the interrenal gland (arrow) of MYCN;Gab2wt compound transgenic fish at 4 weeks postfertilization (wpf). Scale bar, 1 mm.

(C) Kaplan-Meier analysis of the cumulative frequency of neuroblastoma induction over 9 weeks in the indicated stable transgenic zebrafish lines (MYCN-only versus MYCN;Gab2wt, p < 0.0001 by log-rank test).

(D) Kaplan-Meier analysis of the cumulative proportion of MYCN transgenic fish mosaically overexpressing mCherry alone (MYCN;mosaic mCherry) or co-expressing mCherry with Gab2wt (MYCN;mosaic Gab2wt) over 11 weeks. Tumor incidence in fish expressing Gab2wt and mCherry was increased over results for mCherry overexpression alone (p = 0.003 by log-rank test).

(E) Immunoblot of Shp2-Erk pathway components in neuroblastomas isolated from MYCN-only or MYCN;Gab2wt transgenic fish. Total Erk1/2 levels serve as loading controls.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zdf16Tg; zf739Tg (AB) standard conditions Days 21-29 interrenal gland neuroblastoma IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-gab2 WB zdf16Tg (AB) standard conditions Adult Not Detected interrenal gland neuroblastoma
WB zdf16Tg; zf739Tg (AB) standard conditions Adult interrenal gland neuroblastoma
Ab6-mapk WB zdf16Tg (AB) standard conditions Adult interrenal gland neuroblastoma
WB zdf16Tg; zf739Tg (AB) standard conditions Adult interrenal gland neuroblastoma
Phenotype Details
Fish Conditions Stage Phenotype
zdf16Tg (AB) standard conditions Days 21-29 interrenal gland neuroblastoma increased amount, abnormal
zdf16Tg; zf739Tg (AB) standard conditions Days 21-29 interrenal gland neuroblastoma increased amount, exacerbated
Adult interrenal gland neuroblastoma Ab1-gab2 labeling increased amount, abnormal
Adult interrenal gland neuroblastoma ab6-mapk labeling increased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.