FIGURE

Fig. 3

ID

ZDB-FIG-140115-7

Publication

Lafontant et al., 2013 - Cardiac myocyte diversity and a fibroblast network in the junctional region of the zebrafish heart revealed by transmission and serial block-face scanning electron microscopy

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Fig. 3

Immunofluorescence imaging of the zebrafish ventricle junctional region.

(A), staining with TRITC-labeled wheat germ agglutinin (WGA) highlighting cardiac myocytes borders, in conjunction with Hoechst staining, to illustrate the transitional cardiac myocytes (arrows). (B), original monochrome image of the TRITC-WGA stained cell borders containing the two right-most nuclei identified by the arrows in panel A. (C), immunostaining with MEF antibody to label myocyte nuclei and FITC-labeled WGA-stained cell membranes; the arrow identifies a transitional cardiac myocyte. (D), original monochrome image of the FITC-WGA stained cell membranes in panel C around the nucleus identified by the arrow. (E), confocal image of FITC-WGA-stained cardiac myocyte membranes. (F), overlay images of FITC-WGA in panel E with MYH1 (anti-myosin heavy chain-1 antibody) immunostaining, and Hoechst staining identifying transitional cardiac myocytes (arrows). Scale bar, 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One