ZFIN ID: ZDB-FIG-120405-19
Jao et al., 2012 - A zebrafish model of lethal congenital contracture syndrome 1 reveals Gle1 function in spinal neural precursor survival and motor axon arborization. Development (Cambridge, England)   139(7):1316-1326 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 5

Secondary motoneurons are severely reduced in gle1 morphants. (A-F) Confocal images of control (A-C) and gle1 morphant (D-F) zebrafish embryos with motoneurons labeled by mnx1:GFP transgene and zn-8 (Alcama) antibody to reveal somata and fasciculated axons of secondary motoneurons at 56 hpf. gle1 morphants lose most zn-8 immunoreactivity, including the rostrally projecting nerves (asterisks in the control, A-C), but still retain the primary motoneurons (mnx1:GFP+) (D,F). Images are lateral views of the trunk spinal cord with dorsal to top, anterior to left. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
alcama zn-8 ml2Tg control Long-pec secondary motor neuron axon IHC
zn-8 Long-pec secondary motor neuron cell body IHC
zn-8 ml2Tg + MO1-gle1 + MO2-gle1 standard conditions Long-pec Not Detected spinal cord motor neuron IHC
GFP ml2Tg control Long-pec primary motor neuron axon IFL
Long-pec primary motor neuron cell body IFL
Long-pec secondary motor neuron axon IFL
Long-pec secondary motor neuron cell body IFL
ml2Tg + MO1-gle1 + MO2-gle1 standard conditions Long-pec primary motor neuron axon IFL
Long-pec primary motor neuron cell body IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ml2Tg + MO1-gle1 + MO2-gle1 standard conditions Long-pec spinal cord lacks parts or has fewer parts of type secondary motor neuron, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development