FIGURE

Fig. S8

ID
ZDB-FIG-120405-29
Publication
Jao et al., 2012 - A zebrafish model of lethal congenital contracture syndrome 1 reveals Gle1 function in spinal neural precursor survival and motor axon arborization
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Fig. S8

Most gfap:GFP+ radial glia are Sox2+ neural precursors, which are located close to the apical surface and are mutually exclusive with motoneurons. (A-D) Transverse cryosections through the spinal cord of wild-type embryos harboring the gfap:GFP transgene were stained with anti-Sox2 antibody at 24 hpf. Most, if not all, gfap:GFP+ cells (A) are also Sox2+ (B, merged channels in C,D). (E-P) Transverse cryosections through the spinal cord of gle1 morphants harboring the mnx1:TagRFP-T transgene were stained with anti-Sox2 antibody at 36 hpf. Note that a portion of mnx1:TagRFP-T+ motoneurons (arrowheads) are located in close proximity to some of the Sox2+ neural precursors (arrows). However, these two signals do not overlap (merged channels in G,K,O and H,L,P). Nuclei were counterstained by TO-PRO-3. Scale bars: 7.5 μm in D; 10 μm in H,L,P.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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