ZFIN ID: ZDB-FIG-120405-17
Jao et al., 2012 - A zebrafish model of lethal congenital contracture syndrome 1 reveals Gle1 function in spinal neural precursor survival and motor axon arborization. Development (Cambridge, England)   139(7):1316-1326 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 3

Motoneuron phenotypes of gle1–/– mutants. (A-D) Confocal images of live wild-type (A,C) and gle1–/– (B,D) zebrafish embryos with motoneurons labeled by mnx1:GFP transgene at 54 hpf (A,B) and 78 hpf (C,D). Compared with wild-type siblings, gle1–/– embryos have fewer motoneurons in the spinal cord (B, bracket), lack rostrally projecting axons (asterisks in wild type, A) around the horizontal myoseptum, and exhibit disorganized axon arbors and ectopic branching (B,D). The phenotypes are exacerbated with age. Images are lateral views of the trunk spinal cord with dorsal to top, anterior to left. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP gle1hi4161aTg/hi4161aTg; ml2Tg standard conditions Long-pec motor neuron axon IFL
Long-pec motor neuron cell body IFL
Long-pec spinal cord motor neuron IFL
Protruding-mouth motor neuron axon IFL
Protruding-mouth motor neuron cell body IFL
Protruding-mouth spinal cord motor neuron IFL
ml2Tg standard conditions Long-pec motor neuron axon IFL
Long-pec motor neuron cell body IFL
Long-pec spinal cord motor neuron IFL
Protruding-mouth motor neuron axon IFL
Protruding-mouth motor neuron cell body IFL
Protruding-mouth spinal cord motor neuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gle1hi4161aTg/hi4161aTg; ml2Tg standard conditions Long-pec axon extension decreased process quality, abnormal
Long-pec motor neuron axon disorganized, abnormal
Long-pec spinal cord has fewer parts of type motor neuron, abnormal
Protruding-mouth axon extension decreased process quality, abnormal
Protruding-mouth motor neuron axon disorganized, abnormal
Protruding-mouth spinal cord has fewer parts of type motor neuron, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development