ZFIN ID: ZDB-FIG-120329-38
Zhu et al., 2012 - Activated ALK Collaborates with MYCN in Neuroblastoma Pathogenesis. Cancer Cell   21(3):362-373 Full text @ Cancer Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Days 30-44
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 21-29 to Days 45-89

Fig. 6

MYCN Causes Hu+ Cell Hyperplasia in the Interrenal Gland (A) Sagittal sections through the interrenal gland in DβH (top panels), MYCN (middle panels), and MYCN;ALK (lower panels) transgenic fish at 5wpf (dorsal up, anterior left). EGFP, green; Hu, magenta; TH, red. Representative sections through the interrenal gland in DβH fish contain three to five GFP+/Hu+/TH+ sympathetic neuroblasts (arrows) and many GFP+/Hu-/TH+ chromaffin cells (arrowheads). Hu+ cell numbers increase in MYCN and MYCN;ALK fish (brackets), and can be GFP+ and TH+. Dotted lines indicate the head kidney (HK) boundary. Scale bar represents 20 μm. (B) Numbers of Hu+ interrenal gland cells in DβH, ALK, MYCN, and MYCN;ALK transgenic fish at 3, 5, and 7 weeks. Means of Hu+ cell numbers were compared by the two-tailed Wilcoxon signed-rank test. See also Figure S6.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zdf15Tg (AB) standard conditions Days 30-44 interrenal gland chromaffin cell IFL
Days 30-44 interrenal gland neuroblast IFL
zdf16Tg (AB) standard conditions Days 30-44 interrenal gland chromaffin cell IFL
Days 30-44 interrenal gland neuroblast IFL
zdf16Tg; zdf17Tg (AB) standard conditions Days 30-44 interrenal gland chromaffin cell IFL
Days 30-44 interrenal gland neuroblast IFL
th zdf15Tg (AB) standard conditions Days 30-44 interrenal gland chromaffin cell IHC
Days 30-44 interrenal gland neuroblast IHC
zdf16Tg (AB) standard conditions Days 30-44 interrenal gland chromaffin cell IHC
Days 30-44 interrenal gland neuroblast IHC
zdf16Tg; zdf17Tg (AB) standard conditions Days 30-44 interrenal gland chromaffin cell IHC
Days 30-44 interrenal gland neuroblast IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zdf17Tg (AB) standard conditions Days 21-29 - Days 45-89 interrenal gland neuroblast increased amount, abnormal
Days 21-29 - Days 45-89 interrenal gland neuroblastoma present, abnormal
Acknowledgments:
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Reprinted from Cancer Cell, 21(3), Zhu, S., Lee, J.S., Guo, F., Shin, J., Perez-Atayde, A.R., Kutok, J.L., Rodig, S.J., Neuberg, D.S., Helman, D., Feng, H., Stewart, R.A., Wang, W., George, R.E., Kanki, J.P., and Look, A.T., Activated ALK Collaborates with MYCN in Neuroblastoma Pathogenesis, 362-373, Copyright (2012) with permission from Elsevier. Full text @ Cancer Cell