Fig. 4
- ID
- ZDB-FIG-090714-27
- Publication
- Wilkinson et al., 2009 - Hedgehog and Bmp polarize hematopoietic stem cell emergence in the zebrafish dorsal aorta
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bmp4 Is Required for HSC Emergence within the Ventral DA (A–D) runx1 expression was absent from the DA (arrows) in 8/12 bmp4 morphants at 28 hpf (C and D), while the remaining 4/12 exhibited reduced expression compared with uninjected controls (A and B). (E–H) At 28 hpf, 17/21 bmp4 morphants exhibited absent or substantially downregulated cmyb expression in the DA (red arrows). (I and J) At 4 dpf, 27/34 bmp4 morphants exhibited substantial downregulation of ikaros expression within the developing thymi (arrows). The remaining 7/34 exhibited wild-type expression. (K and L) At 4 dpf, 26/32 bmp4 morphants exhibited substantially reduced expression of rag1 in developing thymi (arrows), of which 7 exhibited no rag1 expression. The remaining 6/32 were wild-type. (M and N) Dorsolateral views of the developing pronephros at 5 dpf depicting substantial downregulation of cmyb expression (outlined) in 22/29 embryos, of which 4/29 exhibited no expression. The remaining 7/29 were wild-type. (O) Diagram of zebrafish trunk depicting polarization of the DA by dorsal Hedgehog and ventral Bmp signaling, mirroring the neural tube. |
Reprinted from Developmental Cell, 16(6), Wilkinson, R.N., Pouget, C., Gering, M., Russell, A.J., Davies, S.G., Kimelman, D., and Patient, R., Hedgehog and Bmp polarize hematopoietic stem cell emergence in the zebrafish dorsal aorta, 909-916, Copyright (2009) with permission from Elsevier. Full text @ Dev. Cell